PUBLICATION
The Zebrafish curly fry Is Required for Proper Centrosome and Mitotic Spindle Assembly
- Authors
- Song, M.H., Medley, J.C., Kuwada, J.Y.
- ID
- ZDB-PUB-170512-6
- Date
- 2017
- Source
- Zebrafish 14(4): 311-321 (Journal)
- Registered Authors
- Kuwada, John, Song, Mi Hye
- Keywords
- Danio rerio, bipolar spindle, cell cycle, centrosome, mitosis, proliferation
- MeSH Terms
-
- Animals
- Cell Cycle Checkpoints
- Cell Cycle Proteins/genetics
- Cell Proliferation
- Cells, Cultured
- Centrosome/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Mitosis/physiology*
- Mutation
- Spindle Apparatus/physiology*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 28488934 Full text @ Zebrafish
Citation
Song, M.H., Medley, J.C., Kuwada, J.Y. (2017) The Zebrafish curly fry Is Required for Proper Centrosome and Mitotic Spindle Assembly. Zebrafish. 14(4):311-321.
Abstract
The zebrafish curly fry (cfy) mutation leads to a dramatic increase in mitotic index and cell death starting during neural tube formation. The mutant phenotype is cell autonomous and does not result from defects in apical/basal polarity within the neuroepithelium. The increase in mitotic index could be due to increased proliferation or cell cycle arrest in mitosis. cfy embryos were analyzed to examine these two possibilities. By labeling embryos with a pulse of BrdU and anti-phospho-histone 3 and examining the DNA content by fluorescence activated cell sorting, we show that cfy mutants exhibit no increase in proliferation, but a significant increase in the number of cells arrested in mitosis. Furthermore, time-lapse microscopy in vivo confirmed that a great majority of dividing cells arrest during mitosis and that these mitotically arrested cells die in cfy embryos. Finally, immunostaining and confocal microscopy in cfy mutant embryos revealed that mitotic cells in mutants contain aberrant centrosomes and often exhibit monopolar spindles, thereby leading to mitotic cell cycle arrest. Our results suggest that the cfy gene is required for proper centrosome assembly and mitotic spindle formation, therefore critical for normal cell division.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping