PUBLICATION

Comparison of molecular marker expression in early zebrafish brain development following chronic ethanol or morpholino treatment

Authors
Zhang, C., Boa-Amponsem, O., Cole, G.J.
ID
ZDB-PUB-170512-2
Date
2017
Source
Experimental brain research   235(8): 2413-2423 (Journal)
Registered Authors
Cole, Gregory J., Zhang, Chengjin
Keywords
Ethanol, FASD, Fibroblast growth factor, Retinoic acid, Sonic hedgehog, Zebrafish
MeSH Terms
  • Age Factors
  • Animals
  • Brain/anatomy & histology
  • Brain/drug effects*
  • Brain/embryology
  • Brain/metabolism*
  • Central Nervous System Depressants/pharmacology*
  • Embryo, Mammalian
  • Ethanol/pharmacology*
  • Eye/drug effects
  • Eye/embryology
  • Eye/metabolism
  • Gene Expression Regulation, Developmental/drug effects*
  • In Situ Hybridization
  • Morpholines/pharmacology
  • Tretinoin/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
28493069 Full text @ Exp. Brain Res.
Abstract
This study was undertaken to ascertain whether defined markers of early zebrafish brain development are affected by chronic ethanol exposure or morpholino knockdown of agrin, sonic hedgehog, retinoic acid, and fibroblast growth factors, four signaling molecules that are suggested to be ethanol sensitive. Zebrafish embryos were exposed to 2% ethanol from 6 to 24 hpf or injected with agrin, shha, aldh1a3, or fgf8a morpholinos. In situ hybridization was employed to analyze otx2, pax6a, epha4a, krx20, pax2a, fgf8a, wnt1, and eng2b expression during early brain development. Our results showed that pax6a mRNA expression was decreased in eye, forebrain, and hindbrain of both chronic ethanol exposed and select MO treatments. Epha4a expression in rhombomere R1 boundary was decreased in chronic ethanol exposure and aldh1a3 morphants, lost in fgf8a morphants, but largely unaffected in agrin and shha morphants. Ectopic pax6a and epha4a expression in midbrain was only found in fgf8a morphants. These results suggest that while chronic ethanol induces obvious morphological change in brain architecture, many molecular markers of these brain structures are relatively unaffected by ethanol exposure.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping