PUBLICATION
Transgenic overexpression of BAFF regulates the expression of immune-related genes in zebrafish, Danio rerio
- Authors
- Zhang, L., Liu, C., Zhou, X., Xie, Y., Su, L., Geng, Q., Liu, B., Liu, S.
- ID
- ZDB-PUB-161221-6
- Date
- 2016
- Source
- Journal of genetics 95: 751-760 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- B-Cell Activating Factor/genetics*
- Cell Line
- Cloning, Molecular
- Gene Expression*
- Gene Expression Regulation
- Gene Order
- Genes, Reporter
- Genetic Vectors/genetics
- Humans
- Immunity/genetics*
- Transfection
- Zebrafish/genetics*
- Zebrafish/immunology*
- PubMed
- 27994173 Full text @ J. Genet.
Citation
Zhang, L., Liu, C., Zhou, X., Xie, Y., Su, L., Geng, Q., Liu, B., Liu, S. (2016) Transgenic overexpression of BAFF regulates the expression of immune-related genes in zebrafish, Danio rerio. Journal of genetics. 95:751-760.
Abstract
The B-cell activating factor (BAFF) is a member of tumour necrosis factor (TNF) superfamily that specifically regulates B lymphocyte proliferation and survival. Excess BAFF leads to overproduction of antibodies for secretion, anti-dsDNA antibodies and a lupus-like syndrome in mice. To investigate whether transgenic overexpression of the zebrafish BAFF leads to immunoglobulin changes and/or early maturing of the immune system, a Tol2-GFP-2A-BAFF/His recombinant plasmid was constructed by inserting a 2A peptide between the green fluorescent protein (GFP) and BAFF sequences. Functional GFP and BAFF proteins were expressed separately and confirmed in HeLa cells. The relative expression of immune-related genes (IgLC-1, IgLC-2, IgLC-3, IgD, IgM and IL-4), early lymphoid markers (Ikaros, Rag-1 and TCRAC), and the protooncogene Bcl-2 were evaluated by quantitative polymerase chain reaction (PCR) in F0 founder of transgenic zebrafish juveniles and adults. Ectopic expression of BAFF in adults was confirmed using Western blots and was shown to upregulate IgLC-1, IgLC-2, IgD, IgM, IgZ/T, Ikaros, Rag-1, TCRAC, IL-4 and Bcl-2 expression in juveniles on day 21 and IgLC-1, IgLC-2, IgD, IgM,IgZ/T, Rag-1, TCRAC and Bcl-2 expression in zebrafish three months postfertilization. The relative titers of specific IgM against Edwardsiella tarda WED were assessed using modified enzyme-linked immunosorbent assay (ELISA) with the whole body homogenate of zebrafish and demonstrated a significant increase in BAFF-transgenic group. Therefore, our findings provided novel insight into further exploration of modulating adaptive immunity and studying autoimmune diseases caused by regulating BAFF.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping