PUBLICATION
Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost
- Authors
- Ai, K., Luo, K., Li, Y., Hu, W., Gao, W., Fang, L., Tian, G., Ruan, G., Xu, Q.
- ID
- ZDB-PUB-161120-13
- Date
- 2017
- Source
- Fish & shellfish immunology 60: 59-64 (Journal)
- Registered Authors
- Hu, Wei
- Keywords
- Expression, Interferon regulatory factor 4 (IRF4), Modulation, Paralogues
- MeSH Terms
-
- Animals
- Cell Line
- Fish Diseases/genetics
- Fish Diseases/immunology
- Gene Expression Regulation*
- Immunity, Innate/genetics*
- Interferon Regulatory Factors/genetics*
- Interferon Regulatory Factors/metabolism
- Phylogeny
- Reoviridae/physiology
- Reoviridae Infections/genetics
- Reoviridae Infections/immunology
- Reoviridae Infections/veterinary
- Rhabdoviridae/physiology
- Rhabdoviridae Infections/genetics
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary
- Sequence Analysis, DNA
- Zebrafish/classification
- Zebrafish/genetics*
- Zebrafish/immunology*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 27856326 Full text @ Fish Shellfish Immunol.
Citation
Ai, K., Luo, K., Li, Y., Hu, W., Gao, W., Fang, L., Tian, G., Ruan, G., Xu, Q. (2017) Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost. Fish & shellfish immunology. 60:59-64.
Abstract
In mammals, interferon regulatory factor 4 (IRF4) plays an important role in the process of development and differentiation of B cells, T cells and dendritic cells. It can regulate immune pathway through IRF5, MyD88, IL21, PGC1α, and NOD2. In the present study, we investigated the expression pattern of IRF4 paralogues and these related genes for the first time in teleosts. The results showed that these genes were all expressed predominantly in known immune tissues while IRF5 was also relatively highly expressed in muscle. IRF4b, IL21, MyD88, IRF5 and NOD2 showed maternal expression in the oocyte and the higher expression of IRF4a, Mx and PGC1α before hatching might be involved in the embryonic innate defense system. Zebrafish embryonic fibroblast (ZF4) cells were infected with GCRV and SVCV. During GCRV infection, the expression of Mx was significantly up-regulated from 3 h to 24 h, reaching the highest level at 12 h (101.5-fold over the controls, P < 0.001). And the expression of IRF4a was significantly up-regulated from 3 h to 48 h, reaching the highest level at 12 h (13.75-fold over the controls, P < 0.001). While the expression of IRF4b was only slightly up-regulated at 12 h and 24 h (3.39-fold, 1.93-fold) above control levels, respectively. Whereas the expression of Mx was significantly up-regulated during SVCV infection from 1 h to 48 h, reaching the highest level at 24 h (11.49-fold over the controls, P < 0.001). IRF4a transcripts were significantly up-regulated from 6 h to 24 h, reaching the highest level at 24 h (41-fold over the controls, P < 0.01). IRF4b only showed a slightly up-regulation by SVCV at 24 h (3.2-fold over the controls, P < 0.01). IRF4a and IRF4b displayed a distinct tissue expression pattern, embryonic stages expression and inducible expression in vivo and in vitro, suggesting that IRF4 paralogues might play different roles in immune system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping