PUBLICATION
cDNA cloning and expression analysis of a hepcidin gene from yellow catfish Pelteobagrus fulvidraco (siluriformes: Bagridae)
- Authors
- Liu, Q.N., Xin, Z.Z., Zhang, D.Z., Jiang, S.H., Chai, X.Y., Wang, Z.F., Li, C.F., Zhou, C.L., Tang, B.P.
- ID
- ZDB-PUB-161107-4
- Date
- 2017
- Source
- Fish & shellfish immunology 60: 247-254 (Journal)
- Registered Authors
- Keywords
- Expression analysis, Hepcidin, Immune response, Pelteobagrus fulvidraco
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Catfishes/genetics*
- Catfishes/immunology
- Catfishes/metabolism
- Cloning, Molecular
- DNA, Complementary/genetics
- DNA, Complementary/metabolism
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Gene Expression Profiling
- Gene Expression Regulation*
- Hepcidins/chemistry
- Hepcidins/genetics*
- Hepcidins/metabolism
- Immunity, Innate*
- Lipopolysaccharides/pharmacology
- Phylogeny
- Poly I-C/pharmacology
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction/veterinary
- Sequence Alignment/veterinary
- PubMed
- 27815205 Full text @ Fish Shellfish Immunol.
Citation
Liu, Q.N., Xin, Z.Z., Zhang, D.Z., Jiang, S.H., Chai, X.Y., Wang, Z.F., Li, C.F., Zhou, C.L., Tang, B.P. (2017) cDNA cloning and expression analysis of a hepcidin gene from yellow catfish Pelteobagrus fulvidraco (siluriformes: Bagridae). Fish & shellfish immunology. 60:247-254.
Abstract
Hepcidin is a small, cysteine-rich antimicrobial peptide with a highly conserved β-sheet structure that plays a vital role in innate host immunity against pathogenic organisms. In this study, a hepcidin gene was identified in Pelteobagrus fulvidraco, an economically important freshwater fish in China. The gene is named PfHep. The complete PfHep cDNA was 723 bp, including a 5'-untranslated region (UTR) of 102 bp, a 3'-UTR of 339 bp and an open reading frame of 282 bp encoding a polypeptide of 93 amino acids, which includes a predicted signal peptide and the Hepcidin domain. The predicted mature, cationic PfHep protein has a typical hepcidin RX (K/R)R motif and eight conserved cysteine residues. The deduced PfHep protein sequence has 70%, 54% and 39% percent identity with hepcidins from Ictalurus punctatus, Danio rerio, and Homo sapiens, respectively. The predicted tertiary structure of PfHep is very similar to that of hepcidin in other animals. Phylogenetic analysis revealed that PfHep is closely related to the hepcidins of I. punctatus and I. furcatus. Real-time quantitative reverse transcription-PCR showed that the PfHep gene was expressed most in liver of healthy P. fulvidraco, and expressed to some extent in all the tissues tested. After challenge with lipopolysaccharide and polyriboinosinic:polyribocytidylic acid (poly I:C), respectively, the expression levels of PfHep were markedly upregulated in liver, spleen, head kidney and blood at different time points. Together these results imply that PfHep may be an important component of the innate immune system and be involved in immune defense against invading pathogens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping