PUBLICATION
A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease
- Authors
- Don, E.K., Formella, I., Badrock, A.P., Hall, T.E., Morsch, M., Hortle, E., Hogan, A., Chow, S., Gwee, S.S., Stoddart, J.J., Nicholson, G., Chung, R., Cole, N.J.
- ID
- ZDB-PUB-160916-3
- Date
- 2017
- Source
- Zebrafish 14(1): 69-72 (Journal)
- Registered Authors
- Badrock, Andrew P., Cole, Nicholas, Don, Emily, Hall, Thomas, Stoddart, Jack
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics*
- DNA Transposable Elements*
- DNA, Recombinant/genetics
- Gene Transfer Techniques*
- Genetic Vectors
- Green Fluorescent Proteins/genetics
- Nervous System Diseases/genetics*
- Neurodegenerative Diseases/genetics*
- Promoter Regions, Genetic
- Recombination, Genetic
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 27631880 Full text @ Zebrafish
Citation
Don, E.K., Formella, I., Badrock, A.P., Hall, T.E., Morsch, M., Hortle, E., Hogan, A., Chow, S., Gwee, S.S., Stoddart, J.J., Nicholson, G., Chung, R., Cole, N.J. (2017) A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Zebrafish. 14(1):69-72.
Abstract
Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping