PUBLICATION

The proteins of Vent-family and their mRNAs are located in different areas of the tails of Zebrafish and Xenopus embryos

Authors
Pshennikova, E.S., Voronina, A.S.
ID
ZDB-PUB-160914-5
Date
2016
Source
The international journal of biochemistry & cell biology   79: 388-392 (Journal)
Registered Authors
Keywords
Vox, Xvent-2, mRNP, neural crest, tail bud
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism*
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Protein Transport
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Repressor Proteins/genetics*
  • Repressor Proteins/metabolism*
  • Tail/embryology
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism*
  • Xenopus Proteins/genetics*
  • Xenopus Proteins/metabolism*
  • Xenopus laevis/embryology*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed
27620076 Full text @ Int. J. Biochem. Cell Biol.
Abstract
In molecular embryology, by tacit consent, a presence or absence of the specific mRNA in the cell indicates the presence or absence of the corresponding protein. However, there are lots of evidences that mRNA may be associated with inactive non-translated ribonucleoprotein complexes. Here we for the first time compared the temporal and spatial distribution of the Vent-family transcription factors and their mRNAs in the tails of embryos of two biological species - Xenopus laevis and zebrafish (Danio reria). We have shown that Xvent-2 mRNA was not active in tails of Xenopus embryos at the early tail bud stage. Although the Xvent-2 mRNA of Xenopus and Vox mRNA of zebrafish embryos were detected in the tips of the tails, the Xvent-2 and Vox proteins were revealed in other regions of the tails - along axial structures and around somites. We propose that when the translation of masked Xvent-2 (or Vox) mRNA is activated in a tail bud cell, this transcription factor changes activity of target genes. As a result, the cell comes to differentiation.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping