PUBLICATION
Distinct effects of inflammation on preconditioning and regeneration of the adult zebrafish heart
- Authors
- de Preux Charles, A.S., Bise, T., Baier, F., Marro, J., Jaźwińska, A.
- ID
- ZDB-PUB-160722-5
- Date
- 2016
- Source
- Open Biology 6(7): (Journal)
- Registered Authors
- Jazwinska, Anna
- Keywords
- cardiac muscle, cardiomyocyte, cryoinjury, leucocytes, non-mammalian animal model, thoracotomy
- MeSH Terms
-
- Aminopyridines/pharmacology
- Animals
- Anti-Inflammatory Agents/pharmacology
- Cell Cycle
- Cell Proliferation
- Cryopreservation
- Disease Models, Animal
- Heart/physiology*
- Inflammation/drug therapy
- Inflammation/etiology
- Inflammation/immunology*
- Ischemic Preconditioning, Myocardial/methods*
- Leukocytes/cytology
- Myocardial Infarction/etiology
- Myocardial Infarction/immunology
- Myocardial Infarction/therapy*
- Myocytes, Cardiac/cytology*
- Myocytes, Cardiac/immunology
- Pyrroles/pharmacology
- Regeneration*
- Thoracotomy
- Zebrafish/physiology
- PubMed
- 27440424 Full text @ Open Biol.
Citation
de Preux Charles, A.S., Bise, T., Baier, F., Marro, J., Jaźwińska, A. (2016) Distinct effects of inflammation on preconditioning and regeneration of the adult zebrafish heart. Open Biology. 6(7).
Abstract
The adult heart is able to activate cardioprotective programmes and modifies its architecture in response to physiological or pathological changes. While mammalian cardiac remodelling often involves hypertrophic expansion, the adult zebrafish heart exploits hyperplastic growth. This capacity depends on the responsiveness of zebrafish cardiomyocytes to mitogenic signals throughout their entire life. Here, we have examined the role of inflammation on the stimulation of cell cycle activity in the context of heart preconditioning and regeneration. We used thoracotomy as a cardiac preconditioning model and cryoinjury as a model of cardiac infarction in the adult zebrafish. First, we performed a spatio-temporal characterization of leucocytes and cycling cardiac cells after thoracotomy. This analysis revealed a concomitance between the infiltration of inflammatory cells and the stimulation of the mitotic activity. However, decreasing the immune response using clodronate liposome injection, PLX3397 treatment or anti-inflammatory drugs surprisingly had no effect on the re-entry of cardiac cells into the cell cycle. In contrast, reducing inflammation using the same strategies after cryoinjury strongly impaired cardiac cell mitotic activity and the regenerative process. Taken together, our results show that, while the immune response is not necessary to induce cell-cycle activity in intact preconditioned hearts, inflammation is required for the regeneration of injured hearts in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping