PUBLICATION
Neural Mechanisms Generating Orientation Selectivity in the Retina
- Authors
- Antinucci, P., Suleyman, O., Monfries, C., Hindges, R.
- ID
- ZDB-PUB-160706-11
- Date
- 2016
- Source
- Current biology : CB 26(14): 1802-15 (Journal)
- Registered Authors
- Hindges, Robert
- Keywords
- none
- MeSH Terms
-
- Animals
- Nerve Tissue Proteins/metabolism
- Orientation*
- Retinal Ganglion Cells/physiology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism
- PubMed
- 27374343 Full text @ Curr. Biol.
Citation
Antinucci, P., Suleyman, O., Monfries, C., Hindges, R. (2016) Neural Mechanisms Generating Orientation Selectivity in the Retina. Current biology : CB. 26(14):1802-15.
Abstract
The orientation of visual stimuli is a salient feature of visual scenes. In vertebrates, the first neural processing steps generating orientation selectivity take place in the retina. Here, we dissect an orientation-selective circuit in the larval zebrafish retina and describe its underlying synaptic, cellular, and molecular mechanisms. We genetically identify a class of amacrine cells (ACs) with elongated dendritic arbors that show orientation tuning. Both selective optogenetic ablation of ACs marked by the cell-adhesion molecule Teneurin-3 (Tenm3) and pharmacological interference with their function demonstrate that these cells are critical components for orientation selectivity in retinal ganglion cells (RGCs) by being a source of tuned GABAergic inhibition. Moreover, our morphological analyses reveal that Tenm3(+) ACs and orientation-selective RGCs co-stratify their dendrites in the inner plexiform layer, and that Tenm3(+) ACs require Tenm3 to acquire their correct dendritic stratification. Finally, we show that orientation tuning is present also among bipolar cell presynaptic terminals. Our results define a neural circuit underlying orientation selectivity in the vertebrate retina and characterize cellular and molecular requirements for its assembly.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping