PUBLICATION
Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation
- Authors
- Liu, C.C., Li, L., Lam, Y.W., Siu, C.W., Cheng, S.H.
- ID
- ZDB-PUB-160430-8
- Date
- 2016
- Source
- Scientific Reports 6: 25073 (Journal)
- Registered Authors
- Cheng, Shuk Han
- Keywords
- Cardiovascular biology, Experimental models of disease
- MeSH Terms
-
- Animals
- Arrhythmias, Cardiac/chemically induced
- Arrhythmias, Cardiac/drug therapy
- Arrhythmias, Cardiac/physiopathology
- Calcium Gluconate/administration & dosage
- Calcium Gluconate/therapeutic use
- Disease Models, Animal
- Electrocardiography/methods*
- Heart/physiology*
- Heart Rate
- Injections, Intraperitoneal
- Mutation
- Potassium Chloride/adverse effects
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 27125643 Full text @ Sci. Rep.
Citation
Liu, C.C., Li, L., Lam, Y.W., Siu, C.W., Cheng, S.H. (2016) Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation. Scientific Reports. 6:25073.
Abstract
The adult zebrafish has been used to model the electrocardiogram (ECG) for human cardiovascular studies. Nonetheless huge variations are observed among studies probably because of the lack of a reliable and reproducible recording method. In our study, an adult zebrafish surface ECG recording technique was improved using a multi-electrode method and by pre-opening the pericardial sac. A convenient ECG data analysis method without wavelet transform was also established. Intraperitoneal injection of KCl in zebrafish induced an arrhythmia similar to that of humans, and the arrhythmia was partially rescued by calcium gluconate. Amputation and cryoinjury of the zebrafish heart induced ST segment depression and affected QRS duration after injury. Only cryoinjury decelerated the heart rate. Different changes were also observed in the QT interval during heart regeneration in these two injury models. We also characterized the electrocardiophysiology of breakdance zebrafish mutant with a prolonged QT interval, that has not been well described in previous studies. Our study provided a reliable and reproducible means to record zebrafish ECG and analyse data. The detailed characterization of the cardiac electrophysiology of zebrafish and its mutant revealed that the potential of the zebrafish in modeling the human cardiovascular system exceeds expectations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping