PUBLICATION

Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos

Authors
Nord, H., Dennhag, N., Muck, J., von Hofsten, J.
ID
ZDB-PUB-160408-4
Date
2016
Source
Molecular biology of the cell   27(11): 1853-62 (Journal)
Registered Authors
Muck, Joscha, Nord, Hanna, von Hofsten, Jonas
Keywords
none
MeSH Terms
  • Animals
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Cell Differentiation/genetics
  • Chromatophores
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Melanophores/metabolism
  • Neural Crest/metabolism
  • PAX2 Transcription Factor/genetics*
  • PAX2 Transcription Factor/metabolism*
  • Pigmentation/genetics*
  • Pigmentation/physiology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed
27053658 Full text @ Mol. Biol. Cell
Abstract
The pigment pattern of many animal species is a result of the arrangement of different types of pigment producing chromatophores. The zebrafish have three different types of chromatophores; black melanophores, yellow xanthophores and shimmering iridophores arranged in a characteristic pattern of golden and blue horizontal stripes. In the zebrafish embryo chromatophores derive from the neural crest cells. Using pax7a and pax7b zebrafish mutants we have identified a previously unknown requirement for Pax7 in the xanthophore lineage formation. The absence of Pax7 results in a severe reduction of xanthophore precursor cells and a complete depletion of differentiated xanthophores in embryos as well as adult zebrafish. In contrast, the melanophore lineage is increased in pax7a/pax7b double mutant embryos and larvae, whereas juvenile and adult pax7a/pax7b double mutant zebrafish display a severe decrease in melanophores and a pigment pattern disorganisation, indicative of a xanthophore deficient phenotype. In summary, we propose a novel role for Pax7 in the early specification of chromatophore precursor cells.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping