PUBLICATION
Intracellular Golgi Complex Organization Reveals Tissue Specific Polarity during Zebrafish Embryogenesis
- Authors
- Sepich, D.S., Solnica-Krezel, L.
- ID
- ZDB-PUB-160405-1
- Date
- 2016
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 245(6): 678-91 (Journal)
- Registered Authors
- Sepich, Diane, Solnica-Krezel, Lilianna
- Keywords
- GM130, Tbx16, Wnt/PCP, chordamesoderm, dispersed
- MeSH Terms
-
- Animals
- Cell Polarity/genetics
- Cell Polarity/physiology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Embryonic Development/genetics
- Embryonic Development/physiology
- Gastrulation/genetics
- Gastrulation/physiology
- Golgi Apparatus/genetics
- Golgi Apparatus/metabolism*
- Notochord/embryology
- Notochord/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 27043944 Full text @ Dev. Dyn.
Citation
Sepich, D.S., Solnica-Krezel, L. (2016) Intracellular Golgi Complex Organization Reveals Tissue Specific Polarity during Zebrafish Embryogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 245(6):678-91.
Abstract
Background Cell polarity is essential for directed migration of mesenchymal cells and morphogenesis of epithelial tissues. Studies in cultured cells indicate that a condensed Golgi Complex (GC) is essential for directed protein trafficking to establish cell polarity underlying directed cell migration. Dynamic changes of the GC intracellular organization during early vertebrate development remain to be investigated.
Results We used antibody labeling and fusion proteins in vivo to study the organization and intracellular placement of the GC during early zebrafish embryogenesis. We found that the GC was dispersed into several puncta containing cis- and trans-Golgi Complex proteins, presumably ministacks, until the end of the gastrula period. By early segmentation stages, the GC condensed in cells of the notochord, adaxial mesoderm and neural plate and its intracellular position became markedly polarized away from borders between these tissues.
Conclusions We find that GC is dispersed in early zebrafish cells, even when cells are engaged in massive gastrulation movements. The GC accumulates into patches in a stage and cell-type specific manner, and becomes polarized away from borders between the embryonic tissues. With respect to tissue borders, intracellular GC polarity in notochord is independent of mature apical/basal polarity, Wnt/PCP or signals from adaxial mesoderm. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping