A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation
- Authors
- Kaufman, C.K., Mosimann, C., Fan, Z.P., Yang, S., Thomas, A.J., Ablain, J., Tan, J.L., Fogley, R.D., van Rooijen, E., Hagedorn, E.J., Ciarlo, C., White, R.M., Matos, D.A , Puller, A.C., Santoriello, C., Liao, E.C., Young, R.A., and Zon, L.I.
- ID
- ZDB-PUB-160129-18
- Date
- 2016
- Source
- Science (New York, N.Y.) 351(6272): aad2197 (Journal)
- Registered Authors
- Liao, Eric, Mosimann, Christian, Santoriello, Cristina, van Rooijen, Ellen, White, Richard M., Zon, Leonard I.
- Keywords
- none
- Datasets
- GEO:GSE75355, GEO:GSE61823, GEO:GSE75353, GEO:GSE75356, GEO:GSE75351
- MeSH Terms
-
- Melanoma/genetics*
- Gene Expression Regulation, Developmental*
- Zebrafish*
- Tumor Suppressor Protein p53/genetics
- Green Fluorescent Proteins/genetics
- Embryonic Stem Cells/metabolism
- Enhancer Elements, Genetic
- Proto-Oncogene Proteins B-raf/genetics
- Melanocytes/metabolism
- Animals
- Nerve Tissue Proteins/genetics
- Gene Expression Regulation, Neoplastic*
- Melanoma, Experimental/genetics*
- SOXE Transcription Factors/genetics
- Neural Crest/metabolism*
- Skin Neoplasms/genetics*
- Zebrafish Proteins/genetics
- Animals, Genetically Modified
- Carcinogenesis/genetics*
- Mutation
- Genes, Reporter
- PubMed
- 26823433 Full text @ Science
The “cancerized field” concept posits that cancer-prone cells in a given tissue share an oncogenic mutation, but only discreet clones within the field initiate tumors. Most benign nevi carry oncogenic BRAFV600E mutations but rarely become melanoma. The zebrafish crestin gene is expressed embryonically in neural crest progenitors (NCPs) and specifically reexpressed in melanoma. Live imaging of transgenic zebrafish crestin reporters shows that within a cancerized field (BRAFV600E-mutant; p53-deficient), a single melanocyte reactivates the NCP state, revealing a fate change at melanoma initiation in this model. NCP transcription factors, including sox10, regulate crestin expression. Forced sox10 overexpression in melanocytes accelerated melanoma formation, which is consistent with activation of NCP genes and super-enhancers leading to melanoma. Our work highlights NCP state reemergence as a key event in melanoma initiation.