PUBLICATION
Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function
- Authors
- Houbrechts, A.M., Vergauwen, L., Bagci, E., Van Houcke, J., Heijlen, M., Kulemeka, B., Hyde, D.R., Knapen, D., Darras, V.M.
- ID
- ZDB-PUB-160124-2
- Date
- 2016
- Source
- Molecular and Cellular Endocrinology 424: 81-93 (Journal)
- Registered Authors
- Bagci, Enise, Darras, Veerle, Hyde, David R., Knapen, Dries, Vergauwen, Lucia
- Keywords
- deiodinase, development, morpholino knockdown, retina, thyroid hormone, zebrafish
- MeSH Terms
-
- Animals
- Eye Abnormalities/etiology*
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*
- Gene Knockdown Techniques
- Iodide Peroxidase/genetics*
- Iodide Peroxidase/metabolism
- Organ Size
- Vision, Ocular
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 26802877 Full text @ Mol. Cell. Endocrinol.
Citation
Houbrechts, A.M., Vergauwen, L., Bagci, E., Van Houcke, J., Heijlen, M., Kulemeka, B., Hyde, D.R., Knapen, D., Darras, V.M. (2016) Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function. Molecular and Cellular Endocrinology. 424:81-93.
Abstract
Retinal development in vertebrates relies extensively on thyroid hormones. Their local availability is tightly controlled by several regulators, including deiodinases (Ds). Here we used morpholino technology to explore the roles of Ds during eye development in zebrafish. Transcriptome analysis at 3 days post fertilization (dpf) revealed a pronounced effect of knockdown of both T4-activating Ds (D1D2MO) or knockdown of T3-inactivating D3 (D3bMO) on phototransduction and retinoid recycling. This was accompanied by morphological defects (studied from 1 to 7 dpf) including reduced eye size, disturbed retinal lamination and strong reduction in rods and all four cone types. Defects were more prominent and persistent in D3-deficient fish. Finally, D3-deficient zebrafish larvae had disrupted visual function at 4 dpf and were less sensitive to a light stimulus at 5 dpf. These data demonstrate the importance of TH-activating and -inactivating Ds for correct zebrafish eye development, and point to D3b as a central player.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping