PUBLICATION
Attractant and repellent cues cooperate in guiding a subset of olfactory sensory axons to a well-defined protoglomerular target
- Authors
- Taku, A.A., Marcaccio, C.L., Ye, W., Krause, G.J., Raper, J.A.
- ID
- ZDB-PUB-160107-7
- Date
- 2016
- Source
- Development (Cambridge, England) 143: 123-32 (Journal)
- Registered Authors
- Raper, Jonathan
- Keywords
- Axon guidance, Olfactory development, Protoglomeruli, Sema3D, Semaphorin, Zebrafish
- MeSH Terms
-
- Animals
- Cues
- DNA-Binding Proteins/metabolism
- Gene Expression Regulation, Developmental
- Nerve Growth Factors/genetics
- Nerve Growth Factors/metabolism
- Neuropilin-1/genetics
- Neuropilin-1/metabolism*
- Neuropilin-2/genetics
- Neuropilin-2/metabolism*
- Olfactory Bulb/cytology*
- Olfactory Bulb/growth & development
- Olfactory Pathways/embryology*
- Olfactory Pathways/physiology
- Olfactory Receptor Neurons/cytology*
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism
- Semaphorins/biosynthesis
- Semaphorins/genetics
- Semaphorins/metabolism
- Signal Transduction
- Transcription Factors/metabolism
- Tumor Suppressor Proteins/genetics
- Tumor Suppressor Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 26732841 Full text @ Development
Citation
Taku, A.A., Marcaccio, C.L., Ye, W., Krause, G.J., Raper, J.A. (2016) Attractant and repellent cues cooperate in guiding a subset of olfactory sensory axons to a well-defined protoglomerular target. Development (Cambridge, England). 143:123-32.
Abstract
Olfactory sensory axons target well-defined intermediate targets in the zebrafish olfactory bulb called protoglomeruli well before they form odorant receptor-specific glomeruli. A subset of olfactory sensory neurons are labeled by expression of the or111-7:IRES:GAL4 transgene whose axons terminate in the central zone (CZ) protoglomerulus. Previous work has shown that some of these axons misproject to the more dorsal and anterior dorsal zone (DZ) protoglomerulus in the absence of Netrin 1/Dcc signaling. In search of additional cues that guide these axons to the CZ, we found that Semaphorin 3D (Sema3D) is expressed in the anterior bulb and acts as a repellent that pushes them towards the CZ. Further analysis indicates that Sema3D signaling is mediated through Nrp1a, while Nrp2b also promotes CZ targeting but in a Sema3D-independent manner. nrp1a, nrp2b and dcc transcripts are detected in or111-7 transgene-expressing neurons early in development and both Nrp1a and Dcc act cell-autonomously in sensory neurons to promote accurate targeting to the CZ. dcc and nrp1a double mutants have significantly more DZ misprojections than either single mutant, suggesting that the two signaling systems act independently and in parallel to direct a specific subset of sensory axons to their initial protoglomerular target.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping