PUBLICATION
Probiotic treatment reduces appetite and glucose level in the zebrafish model
- Authors
- Falcinelli, S., Rodiles, A., Unniappan, S., Picchietti, S., Gioacchini, G., Merrifield, D.L., Carnevali, O.
- ID
- ZDB-PUB-160106-12
- Date
- 2016
- Source
- Scientific Reports 6: 18061 (Journal)
- Registered Authors
- Carnevali, Oliana, Unniappan, Suraj
- Keywords
- Metabolic engineering, Microbiome, Transcriptional regulatory elements
- MeSH Terms
-
- Animals
- Appetite*
- Fatty Acids/metabolism
- Gastrointestinal Absorption
- Gastrointestinal Microbiome
- Gastrointestinal Tract/metabolism
- Gastrointestinal Tract/microbiology
- Gastrointestinal Tract/ultrastructure
- Gene Expression
- Glucose/metabolism*
- Larva/genetics
- Larva/metabolism
- Probiotics/administration & dosage*
- Zebrafish/physiology*
- PubMed
- 26727958 Full text @ Sci. Rep.
Citation
Falcinelli, S., Rodiles, A., Unniappan, S., Picchietti, S., Gioacchini, G., Merrifield, D.L., Carnevali, O. (2016) Probiotic treatment reduces appetite and glucose level in the zebrafish model. Scientific Reports. 6:18061.
Abstract
The gut microbiota regulates metabolic pathways that modulate the physiological state of hunger or satiety. Nutrients in the gut stimulate the release of several appetite modulators acting at central and peripheral levels to mediate appetite and glucose metabolism. After an eight-day exposure of zebrafish larvae to probiotic Lactobacillus rhamnosus, high-throughput sequence analysis evidenced the ability of the probiotic to modulate the microbial composition of the gastrointestinal tract. These changes were associated with a down-regulation and up-regulation of larval orexigenic and anorexigenic genes, respectively, an up-regulation of genes related to glucose level reduction and concomitantly reduced appetite and body glucose level. BODIPY-FL-pentanoic-acid staining revealed higher short chain fatty acids levels in the intestine of treated larvae. These results underline the capability of the probiotic to modulate the gut microbiota community and provides insight into how the probiotic interacts to regulate a novel gene network involved in glucose metabolism and appetite control, suggesting a possible role for L. rhamnosus in the treatment of impaired glucose tolerance and food intake disorders by gut microbiota manipulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping