PUBLICATION

Expression dynamics of NADPH oxidases during early zebrafish development

Authors
Weaver, C.J., Fai Leung, Y., Suter, D.M.
ID
ZDB-PUB-151216-4
Date
2016
Source
The Journal of comparative neurology   524(10): 2130-41 (Journal)
Registered Authors
Keywords
NADPH oxidase, expression, nervous system development, zebrafish (RRID:ZIRC_ZL1)
MeSH Terms
  • Animals
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental/physiology*
  • NADPH Oxidases/genetics
  • NADPH Oxidases/metabolism*
  • RNA, Messenger/metabolism
  • Zebrafish*/embryology
  • Zebrafish*/growth & development
  • Zebrafish*/metabolism
PubMed
26662995 Full text @ J. Comp. Neurol.
Abstract
Nicotinamide dinucleotide phosphate oxidases (NOX) control various cellular signaling cascades. In the nervous system, there is recent evidence that NOX-derived reactive oxygen species (ROS) regulate neurite outgrowth, regeneration, and stem cell proliferation; however, a comprehensive NOX gene expression analysis is missing for all major model systems. Zebrafish embryos provide an excellent model system to study neurodevelopment and regeneration because they develop quickly and are well suited for in vivo imaging and molecular approaches. Although the sequences of five NOX genes (nox1, nox2/cybb, nox4, nox5, and duox) have been identified in the zebrafish genome, nothing is known about their expression pattern. Here, we used quantitative PCR combined with in situ hybridization to develop a catalog of nox1, nox2/cybb, nox5, and duox expression in zebrafish during early nervous system development from 12 to 48 h post-fertilization. We found that expression levels of nox1, nox5, and duox are dynamic during the first 2 days of development, whereas nox2/cybb levels remain remarkably stable. By sectioning in situ hybridized embryos, we found a pattern of broad and overlapping NOX isoform expression at 1 and 1.5 days post-fertilization. After 2 days of development, a few brain regions displayed increased NOX expression levels. Collectively, these results represent the first comprehensive analysis of NOX gene expression in the zebrafish and will provide a basis for future studies aimed at determining the functions of NOX enzymes in neurodevelopment and regeneration. This article is protected by copyright. All rights reserved.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping