PUBLICATION
Identification of the neural-crest specific enhancer of Seraf gene in avian peripheral nervous system development
- Authors
- Suzuki, T., Osumi, N., Wakamatsu, Y.
- ID
- ZDB-PUB-151024-6
- Date
- 2015
- Source
- Biochemical and Biophysical Research Communications 467(4): 1103-9 (Journal)
- Registered Authors
- Keywords
- Neural crest, Schwann cell, Seraf, Sox10, enhancer, neuregulin
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Birds/genetics*
- DNA
- Molecular Sequence Data
- Neural Crest/metabolism*
- Peripheral Nervous System/metabolism*
- Sequence Homology, Amino Acid
- PubMed
- 26494297 Full text @ Biochem. Biophys. Res. Commun.
Citation
Suzuki, T., Osumi, N., Wakamatsu, Y. (2015) Identification of the neural-crest specific enhancer of Seraf gene in avian peripheral nervous system development. Biochemical and Biophysical Research Communications. 467(4):1103-9.
Abstract
In vertebrate embryos, trunk neural crest cells give rise to Schwann cells, along with other derivatives. In this study, to elucidate the molecular mechanisms for the Schwann cell specification, we aimed to identify enhancer elements responsible for the expression of the Seraf gene, the earliest marker for the Schwann cell precursors in the avian embryos. We first compared the genomic structure around the Seraf locus in various vertebrates, and found that, while mammals do not have a Seraf homolog, teleost fish species have it. However, the intergenic sequences around the Seraf locus are not conserved between zebrafish and chicken, consistent with the fact that fish Seraf expression is not Schwann cell precursor-specific. We thus compared the intergenic sequences around the Seraf locus among avian species, and identified a potential enhancer containing a cluster of Sox10-binding sites. Accordingly, the identified enhancer is activated in a neural crest-specific manner in transfected quail embryos. We also found that Sox10 activated the enhancer in cultured cells. Thus, our results revealed a new role of Sox10 in the earliest phase of the Schwann cell fate specification.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping