PUBLICATION
The zebrafish miR-462/miR-731 cluster is induced under hypoxic stress via hypoxia-inducible factor 1α and functions in cellular adaptations
- Authors
- Huang, C.X., Chen, N., Wu, X.J., Huang, C.H., He, Y., Tang, R., Wang, W.M., Wang, H.L.
- ID
- ZDB-PUB-150813-7
- Date
- 2015
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29(12): 4901-13 (Journal)
- Registered Authors
- Keywords
- cell survival, embryonic development, hypoxamir, low oxygen
- MeSH Terms
-
- Adaptation, Physiological*
- Animals
- Cell Line
- Humans
- Hypoxia/genetics*
- Hypoxia-Inducible Factor 1, alpha Subunit/physiology*
- MicroRNAs/genetics*
- Multigene Family
- Up-Regulation/physiology
- Zebrafish
- PubMed
- 26265472 Full text @ FASEB J.
Citation
Huang, C.X., Chen, N., Wu, X.J., Huang, C.H., He, Y., Tang, R., Wang, W.M., Wang, H.L. (2015) The zebrafish miR-462/miR-731 cluster is induced under hypoxic stress via hypoxia-inducible factor 1α and functions in cellular adaptations. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(12):4901-13.
Abstract
Hypoxia, a unique and essential environmental stress, evokes highly coordinated cellular responses, and hypoxia-inducible factor (HIF) 1 in the hypoxia signaling pathway, an evolutionarily conserved cellular signaling pathway, acts as a master regulator of the transcriptional response to hypoxic stress. MicroRNAs (miRNAs), a major class of posttranscriptional gene expression regulators, also play pivotal roles in orchestrating hypoxia-mediated cellular adaptations. Here, global miRNA expression profiling and quantitative real-time PCR indicated that the up-regulation of the miR-462/miR-731 cluster in zebrafish larvae is induced by hypoxia. It was further validated that miR-462 and miR-731 are up-regulated in a Hif-1α-mediated manner under hypoxia and specifically target ddx5 and ppm1da, respectively. Overexpression of miR-462 and miR-731 represses cell proliferation through blocking cell cycle progress of DNA replication, and induces apoptosis. In situ detection revealed that the miR-462/miR-731 cluster is highly expressed in a consistent and ubiquitous manner throughout the early developmental stages. Additionally, the transcripts become restricted to the notochord, pharyngeal arch, liver, and gut regions from postfertiliztion d 3 to 5. These data highlight a previously unidentified role of the miR-462/miR-731 cluster as a crucial signaling mediator for hypoxia-mediated cellular adaptations and provide some insights into the potential function of the cluster during embryonic development.-Huang, C.-X., Chen, N., Wu, X.-J., Huang, C.-H., He, Y., Tang, R., Wang, W.-M., Wang, H.-L. The zebrafish miR-462/miR-731 cluster is induced under hypoxic stress via hypoxia-inducible factor 1α and functions in cellular adaptations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping