PUBLICATION
Neuronal Connectivity between Habenular Glutamate-Kisspeptin1 Co-expressing Neurons and the Raphe 5-HT System
- Authors
- Nathan, F.M., Ogawa, S., Parhar, I.S.
- ID
- ZDB-PUB-150808-4
- Date
- 2015
- Source
- Journal of neurochemistry 135(4): 814-29 (Journal)
- Registered Authors
- Ogawa, Satoshi
- Keywords
- GPR54, Kiss1, serotonin, ventro-anterior MR, zebrafish
- MeSH Terms
-
- Raphe Nuclei/cytology*
- Raphe Nuclei/metabolism
- Glutamic Acid/metabolism*
- Animals
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Serotonin/metabolism*
- RNA, Messenger/metabolism
- Transcription Factor Brn-3A/genetics
- Molecular Sequence Data
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Habenula/cytology*
- Habenula/metabolism
- Nerve Net/metabolism
- Kisspeptins/metabolism*
- Animals, Genetically Modified
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Neurons/metabolism*
- Glutamate Decarboxylase/genetics
- Receptors, G-Protein-Coupled/metabolism
- Male
- PubMed
- 26250886 Full text @ J. Neurochem.
Citation
Nathan, F.M., Ogawa, S., Parhar, I.S. (2015) Neuronal Connectivity between Habenular Glutamate-Kisspeptin1 Co-expressing Neurons and the Raphe 5-HT System. Journal of neurochemistry. 135(4):814-29.
Abstract
The habenula, located on the dorsal thalamic surface, is an emotional and reward processing center. As in the mammalian brain, the zebrafish habenula is divided into dorsal (dHb) and ventral (vHb) subdivisions that project to the interpeduncular nucleus (IPN) and median raphe (MR) respectively. Previously, we have shown that kisspeptin 1 (Kiss1) expressing in the vHb, regulates the serotonin (5-HT) system in the MR. However, the connectivity between the Kiss1 neurons and the 5-HT system remains unknown. To resolve this issue, we generated a specific antibody against zebrafish Kiss1 receptor (Kiss-R1); using this primary antibody we found intense immunohistochemical labeling in the ventro-anterior corner of the MR (vaMR) but not in 5-HT neurons, suggesting the potential involvement of interneurons in 5-HT modulation by Kiss1. Double-fluorescence labeling showed that the majority of habenular Kiss1 neurons are glutamatergic. In the MR region, Kiss1 fibers were mainly seen in close association with glutamatergic neurons and only scarcely within GABAergic and 5-HT neurons. Our findings indicate that the habenular Kiss1 neurons potentially modulate the 5-HT system primarily through glutamatergic neurotransmission via as yet uncharacterized interneurons. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping