PUBLICATION
Control of tissue growth by Yap relies on cell density and F-actin in zebrafish fin regeneration
- Authors
- Mateus, R., Lourenço, R., Fang, Y., Brito, G., Farinho, A., Valério, F., Jacinto, A.
- ID
- ZDB-PUB-150726-3
- Date
- 2015
- Source
- Development (Cambridge, England) 142(16): 2752-63 (Journal)
- Registered Authors
- Fang, Yi, Lourenço, Raquel, Mateus, Rita
- Keywords
- Zebrafish, Fin regeneration, Hippo/Yap, F-actin, Cell density
- MeSH Terms
-
- Actins/metabolism*
- Animal Fins/metabolism
- Animal Fins/physiology*
- Animals
- Cell Count
- Cell Proliferation/physiology*
- Cytoskeleton/physiology
- Image Processing, Computer-Assisted
- In Situ Hybridization
- Microscopy, Fluorescence
- Oligonucleotides, Antisense/genetics
- Real-Time Polymerase Chain Reaction
- Regeneration/physiology*
- Signal Transduction/physiology*
- Trans-Activators/metabolism*
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism*
- PubMed
- 26209644 Full text @ Development
Citation
Mateus, R., Lourenço, R., Fang, Y., Brito, G., Farinho, A., Valério, F., Jacinto, A. (2015) Control of tissue growth by Yap relies on cell density and F-actin in zebrafish fin regeneration. Development (Cambridge, England). 142(16):2752-63.
Abstract
Caudal fin regeneration is characterized by a proliferation boost in the mesenchymal blastema controlled precisely in time and space. This allows a gradual and robust restoration of original fin size. However, how this is established and regulated is not well understood. Here we report that Yap, the Hippo pathway effector, is a chief player in this process: functionally manipulating Yap during regeneration dramatically affects cell proliferation and expression of key signaling pathways, impacting regenerative growth. The intracellular location of Yap is tightly associated with different cell densities along the blastema proximal-distal axis, which correlate with alterations in cell morphology, cytoskeleton and cell-cell contacts in a gradient-like manner. Importantly, Yap inactivation occurs in high cell density areas, conditional to F-actin distribution and polymerization. We propose that Yap is essential for fin regeneration and its function is dependent on mechanical tension, conferred by a balancing act of cell density and cytoskeleton activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping