PUBLICATION
Annexin A3 Regulates Early Blood Vessel Formation
- Authors
- Meadows, S.M., Cleaver, O.
- ID
- ZDB-PUB-150717-1
- Date
- 2015
- Source
- PLoS One 10: e0132580 (Journal)
- Registered Authors
- Keywords
- Embryos, Blood vessels, In situ hybridization, Small interfering RNAs, Xenopus, Frogs, Green fluorescent protein, Zebrafish
- MeSH Terms
-
- Animals
- Annexin A3/antagonists & inhibitors
- Annexin A3/genetics*
- Annexin A3/metabolism
- Cell Death
- Cell Line
- Cell Proliferation
- Embryo, Mammalian
- Embryo, Nonmammalian
- Endothelial Cells/cytology
- Endothelial Cells/metabolism
- Female
- Gene Expression Regulation, Developmental*
- Male
- Mice
- Mice, Transgenic
- Microinjections
- Morpholinos/genetics
- Morpholinos/metabolism
- Neovascularization, Physiologic/genetics*
- Xenopus laevis
- Zebrafish
- PubMed
- 26182056 Full text @ PLoS One
Citation
Meadows, S.M., Cleaver, O. (2015) Annexin A3 Regulates Early Blood Vessel Formation. PLoS One. 10:e0132580.
Abstract
Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (Anxa3) is largely unknown. Our studies identify Anxa3 as a unique marker of the endothelial and myeloid cell lineages of Xenopus laevis during development. Anxa3 transcripts are also detected in endothelial cells (ECs) of zebrafish and mouse embryos, suggesting an important evolutionary function during formation of blood vessels. Indeed, Anxa3 loss-of-function experiments in frog embryos reveal its critical role during the morphogenesis of early blood vessels, as angioblasts in MO injected embryos fail to form vascular cords. Furthermore, in vitro experiments in mammalian cells identify a role for Anxa3 in EC migration. Our results are the first to reveal an in vivo function for Anxa3 during vascular development and represent a previously unexplored aspect of annexin biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping