PUBLICATION
Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio
- Authors
- Morris, J.K., Chomyk, A., Song, P., Parker, N., Deckard, S., Trapp, B.D., Pimplikar, S.W., Dutta, R.
- ID
- ZDB-PUB-150711-3
- Date
- 2015
- Source
- Invertebrate neuroscience : IN 15: 180 (Journal)
- Registered Authors
- Parker, Nathan, Song, Ping
- Keywords
- Danio rerio, miR-124, Neuron, Oligodendrocyte
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/physiology*
- Larva
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- Morpholinos/pharmacology
- Oligodendroglia/drug effects
- Oligodendroglia/metabolism*
- Zebrafish/anatomy & histology*
- PubMed
- 26159098 Full text @ Invert. Neurosci.
Citation
Morris, J.K., Chomyk, A., Song, P., Parker, N., Deckard, S., Trapp, B.D., Pimplikar, S.W., Dutta, R. (2015) Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio. Invertebrate neuroscience : IN. 15:180.
Abstract
Oligodendrocytes produce multi-lamellar myelin membranes that surround axons in the central nervous system (CNS). Preservation and generation of myelin are potential therapeutic targets for dysmyelinating and demyelinating diseases. MicroRNAs (miRNAs) play a vital role in oligodendrocyte differentiation and overall CNS development. miR-124 is a well-conserved neuronal miRNA with important roles in neuronal differentiation and function. miR-124 levels increase following loss of myelin in both human and rodent brains. While the role of neuronal miR-124 in neurogenesis has been established, its effects on axonal outgrowth and oligodendrocytes are not currently known. We therefore explored the possible effect of selective knockdown of miR-124 in Danio rerio using a morpholino-based knockdown approach. No morphological abnormalities or loss of motor neurons were detected despite loss of axonal outgrowth. Morpholino-based knockdown of miR-124 led to reciprocal increases in mRNA levels of target genes that inhibit axonal and dendritic projections. Importantly, loss of miR-124 led to decreased oligodendrocyte cell numbers and myelination of axonal projections in the ventral hindbrain. Taken together, our results add a new dimension to the existing complexity of neuron-glial relationships and highlight the utility of Danio rerio as a model system to investigate such interactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping