PUBLICATION

Identification of polarized macrophage subsets in zebrafish

Authors
Nguyen Chi, M., Laplace-Builhe, B., Travnickova, J., Luz-Crawford, P., Tejedor, G., Phan, Q.T., Duroux-Richard, I., Levraud, J.P., Kissa, K., Lutfalla, G., Jorgensen, C., Djouad, F.
ID
ZDB-PUB-150711-11
Date
2015
Source
eLIFE   4: e07288 (Journal)
Registered Authors
Djouad, Farida, Kissa-Marin, Karima, Levraud, Jean-Pierre, Lutfalla, Georges, Phan, Quang Tien
Keywords
developmental biology, immunology, live imaging, macrophages, stem cells, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Escherichia coli Infections/immunology
  • Flow Cytometry
  • Gene Expression Profiling
  • Genes, Reporter
  • Macrophages/classification*
  • Macrophages/immunology*
  • Microscopy, Confocal
  • Molecular Sequence Data
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Tumor Necrosis Factor-alpha/biosynthesis
  • Wounds and Injuries/immunology
  • Zebrafish/immunology*
PubMed
26154973 Full text @ Elife
Abstract
While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tnfa, a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and E. coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on FACS-sorted tnfa+ and tnfa- macrophages showed that they respectively expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa+ macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping