PUBLICATION
Histological Characterization of the Dicer1 Mutant Zebrafish Retina
- Authors
- Akhtar, S., Patnaik, S.R., Kotapati Raghupathy, R., Al-Mubrad, T.M., Craft, J.A., Shu, X.
- ID
- ZDB-PUB-150701-4
- Date
- 2015
- Source
- Journal of ophthalmology 2015: 309510 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 26124959 Full text @ J Ophthalmol
Citation
Akhtar, S., Patnaik, S.R., Kotapati Raghupathy, R., Al-Mubrad, T.M., Craft, J.A., Shu, X. (2015) Histological Characterization of the Dicer1 Mutant Zebrafish Retina. Journal of ophthalmology. 2015:309510.
Abstract
DICER1, a multidomain RNase III endoribonuclease, plays a critical role in microRNA (miRNA) and RNA-interference (RNAi) functional pathways. Loss of Dicer1 affects different developmental processes. Dicer1 is essential for retinal development and maintenance. DICER1 was recently shown to have another function of silencing the toxicity of Alu RNAs in retinal pigment epithelium (RPE) cells, which are involved in the pathogenesis of age related macular degeneration. In this study, we characterized a Dicer1 mutant fish line, which carries a nonsense mutation (W1457Ter) induced by N-ethyl-N-nitrosourea mutagenesis. Zebrafish DICER1 protein is highly conserved in the evolution. Zebrafish Dicer1 is expressed at the earliest stages of zebrafish development and persists into late developmental stages; it is widely expressed in adult tissues. Homozygous Dicer1 mutant fish (DICER1(W1457Ter/W1457Ter)) have an arrest in early growth with significantly smaller eyes and are dead at 14-18 dpf. Heterozygous Dicer1 mutant fish have similar retinal structure to that of control fish; the retinal pigment epithelium (RPE) cells are normal with no sign of degeneration at the age of 20 months.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping