PUBLICATION
Analysis of nicastrin gene phylogeny and expression in zebrafish
- Authors
- Lim, A., Moussavi Nik, S.H., Ebrahimie, E., Lardelli, M.
- ID
- ZDB-PUB-150506-5
- Date
- 2015
- Source
- Development genes and evolution 225(3): 171-8 (Journal)
- Registered Authors
- Lardelli, Michael
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Amyloid Precursor Protein Secretases/chemistry
- Amyloid Precursor Protein Secretases/genetics*
- Amyloid Precursor Protein Secretases/metabolism*
- Animals
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Membrane Glycoproteins/chemistry
- Membrane Glycoproteins/genetics*
- Membrane Glycoproteins/metabolism*
- Molecular Sequence Data
- Phylogeny*
- Real-Time Polymerase Chain Reaction
- Sequence Alignment
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 25940938 Full text @ Dev. Genes Evol.
Citation
Lim, A., Moussavi Nik, S.H., Ebrahimie, E., Lardelli, M. (2015) Analysis of nicastrin gene phylogeny and expression in zebrafish. Development genes and evolution. 225(3):171-8.
Abstract
NICASTRIN is a component of the aspartyl protease γ-secretase complex which is involved in intramembranous cleavage of type I transmembrane proteins, notably the Notch receptor proteins and the AMYLOID BETA A4 PRECURSOR PROTEIN (APP). This study aimed to characterize the orthologue of the human NICASTRIN (NCSTN) gene in zebrafish, an advantageous model organism for the study of human disease. Zebrafish Nicastrin protein was predicted to possess the conserved glutamate 333 residue and DYIGS motif of human NCSTN that are important for substrate recognition/processing in γ-secretase. Quantitative real-time RT-PCR revealed the profile of relative zebrafish nicastrin (ncstn) transcript levels in embryos at different times during development and in adult tissues. The analysis of synteny conservation revealed local rearrangements of ncstn and another gene, mpz, relative to copa, and pex19. In situ hybridization showed higher relative levels of ncstn transcripts in the developing brain and otic vesicles of embryos at 24 and 48 h post fertilization, respectively. Our observations are consistent with a role for Ncstn protein in Notch signaling within the proliferative ventricular zone of the developing central nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping