PUBLICATION
Zebrafish Nk-lysins: first insights about their cellular and functional diversification
- Authors
- Pereiro, P., Varela, M., Diaz-Rosales, P., Romero, A., Dios, S., Figueras, A., Novoa, B.
- ID
- ZDB-PUB-150331-25
- Date
- 2015
- Source
- Developmental and comparative immunology 51(1): 148-59 (Journal)
- Registered Authors
- Figueras, Antonio, Novoa, Beatriz
- Keywords
- Granulysin, Nk-lysin, Ontogeny, Rag1, SVCV, Zebrafish
- MeSH Terms
-
- Animals
- Antigens, Differentiation, T-Lymphocyte/genetics
- Base Sequence
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Gene Expression Regulation
- Genes, RAG-1/genetics
- Humans
- Killer Cells, Natural/immunology*
- Killer Cells, Natural/virology
- Molecular Sequence Data
- Mutation/genetics
- Organ Specificity
- Phylogeny
- Proteolipids/genetics
- Proteolipids/metabolism*
- Rhabdoviridae/immunology*
- Rhabdoviridae Infections/immunology*
- T-Lymphocytes, Cytotoxic/immunology*
- T-Lymphocytes, Cytotoxic/virology
- Transcriptome
- Zebrafish/immunology*
- PubMed
- 25813149 Full text @ Dev. Comp. Immunol.
Citation
Pereiro, P., Varela, M., Diaz-Rosales, P., Romero, A., Dios, S., Figueras, A., Novoa, B. (2015) Zebrafish Nk-lysins: first insights about their cellular and functional diversification. Developmental and comparative immunology. 51(1):148-59.
Abstract
Nk-lysins are antimicrobial proteins produced by cytotoxic T lymphocytes and natural killer cells with a broad antimicrobial spectrum (including bacteria, fungi and parasites). Nevertheless, the implication of these proteins in the protection against viral infections is still poorly understood. In this work, four different Nk-lysin genes (nkla, nklb, nklc and nkld) were identified in the zebrafish genome. That means that zebrafish is the species with the higher repertoire of Nk-lysin genes described so far. The differential expression pattern of the Nk-lysins in several tissues, during ontogeny, among the different kidney cell populations, as well as between Rag1(-/-) and Rag1(+/+) individuals, could suggest a certain specialization of different cell types in the production of different Nk-lysin. Moreover, only two of these genes (nkla and nkld) were significantly up-regulated after viral infection, and this observation could be also a consequence of a functional diversification of the zebrafish Nk-lysins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping