PUBLICATION
Osmotic surveillance mediates rapid wound closure through nucleotide release
- Authors
- Gault, W.J., Enyedi, B., Niethammer, P.
- ID
- ZDB-PUB-141224-15
- Date
- 2014
- Source
- The Journal of cell biology 207: 767-82 (Journal)
- Registered Authors
- Enyedi, Balázs, Gault, William, Niethammer, Philipp
- Keywords
- none
- MeSH Terms
-
- Adenosine Triphosphate/metabolism*
- Animals
- Cell Movement
- Epidermis/physiopathology
- Epithelial Cells/physiology
- Extracellular Fluid/physiology
- Hydrolysis
- Larva
- Osmoregulation*
- Wound Healing*
- Zebrafish
- PubMed
- 25533845 Full text @ J. Cell Biol.
Citation
Gault, W.J., Enyedi, B., Niethammer, P. (2014) Osmotic surveillance mediates rapid wound closure through nucleotide release. The Journal of cell biology. 207:767-82.
Abstract
Osmotic cues from the environment mediate rapid detection of epithelial breaches by leukocytes in larval zebrafish tail fins. Using intravital luminescence and fluorescence microscopy, we now show that osmolarity differences between the interstitial fluid and the external environment trigger ATP release at tail fin wounds to initiate rapid wound closure through long-range activation of basal epithelial cell motility. Extracellular nucleotide breakdown, at least in part mediated by ecto-nucleoside triphosphate diphosphohydrolase 3 (Entpd3), restricts the range and duration of osmotically induced cell migration after injury. Thus, in zebrafish larvae, wound repair is driven by an autoregulatory circuit that generates pro-migratory tissue signals as a function of environmental exposure of the inside of the tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping