PUBLICATION
Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway
- Authors
- Lu, F.I., Sun, Y.H., Wei, C.Y., Thisse, C., Thisse, B.
- ID
- ZDB-PUB-141106-9
- Date
- 2014
- Source
- Nature communications 5: 5368 (Journal)
- Registered Authors
- Lu, Fu-I, Sun, Yonghua, Thisse, Bernard, Thisse, Christine, Wei, Changyong
- Keywords
- Cell signalling, Developmental biology
- MeSH Terms
-
- Animals
- Co-Repressor Proteins/metabolism
- Cytoskeletal Proteins/metabolism*
- Gastrula/metabolism
- Mesoderm/metabolism*
- Repressor Proteins/physiology*
- Signal Transduction
- Transcription Factor 7-Like 1 Protein/metabolism*
- Wnt Proteins/metabolism*
- Zebrafish
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology*
- beta Catenin/metabolism*
- PubMed
- 25371059 Full text @ Nat. Commun.
Citation
Lu, F.I., Sun, Y.H., Wei, C.Y., Thisse, C., Thisse, B. (2014) Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway. Nature communications. 5:5368.
Abstract
Upon stimulation by Wnt ligands, the canonical Wnt/β-catenin signalling pathway results in the stabilization of β-catenin and its translocation into the nucleus to form transcriptionally active complexes with sequence-specific DNA-binding T-cell factor/lymphoid enhancer factor (TCF/LEF) family proteins. In the absence of nuclear β-catenin, TCF proteins act as transcriptional repressors by binding to Groucho/Transducin-Like Enhancer of split (TLE) proteins that function as co-repressors by interacting with histone deacetylases whose activity leads to the generation of transcriptionally silent chromatin. Here we show that the transcription factor Ladybird homeobox 2 (Lbx2) positively controls the Wnt/β-catenin signalling pathway in the posterior lateral and ventral mesoderm of the zebrafish embryo at the gastrula stage, by directly interfering with the binding of Groucho/TLE to TCF, thereby preventing formation of transcription repressor complexes. These findings reveal a novel level of regulation of the canonical Wnt/β-catenin signalling pathway occurring in the nucleus and involving tissue-specific derepression of TCF by Lbx2.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping