PUBLICATION
Schwann cells and deleted in colorectal carcinoma direct regenerating motor axons towards their original path
- Authors
- Rosenberg, A.F., Isaacman-Beck, J., Franzini-Armstrong, C., Granato, M.
- ID
- ZDB-PUB-141031-9
- Date
- 2014
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 34: 14668-81 (Journal)
- Registered Authors
- Granato, Michael, Rosenberg, Allison
- Keywords
- DCC, Schwann cell, peripheral nerve, regeneration, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/metabolism*
- Cell Movement/physiology*
- Cell Proliferation/physiology
- Growth Cones/metabolism
- Motor Neurons/metabolism*
- Nerve Regeneration/physiology*
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism*
- Schwann Cells/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 25355219 Full text @ J. Neurosci.
Citation
Rosenberg, A.F., Isaacman-Beck, J., Franzini-Armstrong, C., Granato, M. (2014) Schwann cells and deleted in colorectal carcinoma direct regenerating motor axons towards their original path. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34:14668-81.
Abstract
After complete nerve transection, a major challenge for regenerating peripheral axons is to traverse the injury site and navigate toward their original trajectory. Denervated Schwann cells distal to the lesion site secrete factors promoting axonal growth and serve as an axonal substrate, yet whether Schwann cells also actively direct axons toward their original trajectory is unclear. Using live-cell imaging in zebrafish, we visualize for the first time how in response to nerve transection distal Schwann cells change morphology as axons fragment, and how Schwann cell morphology reverses once regenerating growth cones have crossed the injury site and have grown along distal Schwann cells outlining the original nerve path. In mutants lacking Schwann cells, regenerating growth cones extend at rates comparable with wild type yet frequently fail to cross the injury site and instead stray along aberrant trajectories. Providing growth-permissive yet Schwann cell-less scaffolds across the injury site was insufficient to direct regenerating growth cones toward the original path, providing compelling evidence that denervated Schwann cells actively direct regenerating axons across the injury site toward their original trajectory. To identify signals that guide regenerating axons in vivo, we examined mutants lacking the deleted in colorectal carcinoma (DCC) guidance receptor. In these dcc mutants, a significant fraction of regenerating motor axons extended along aberrant trajectories, similar to what we observe in mutants lacking Schwann cells. Thus, Schwann cell and dcc-mediated guidance are critical early during regeneration to direct growth cones across the transection gap and onto their original axonal trajectory.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping