PUBLICATION
Functional Zebrafish Studies Based on Human Genotyping Point to Netrin-1 as a Link Between Aberrant Cardiovascular Development and Thyroid Dysgenesis
- Authors
- Opitz, R., Hitz, M.P., Vandernoot, I., Trubiroha, A., Abu-Khudir, R., Samuels, M., Désilets, V., Costagliola, S., Andelfinger, G., Deladoëy, J.
- ID
- ZDB-PUB-141030-1
- Date
- 2015
- Source
- Endocrinology 1(1): 377-88 (Journal)
- Registered Authors
- Costagliola, Sabine, Opitz, Robert, Trubiroha, Achim, Vandernoot, Isabelle
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cardiovascular Abnormalities/genetics*
- Female
- Gene Expression Regulation, Developmental/physiology*
- Gene Knockdown Techniques
- Genetic Predisposition to Disease
- Genotype*
- Humans
- Male
- Morpholinos
- Nerve Growth Factors/genetics
- Nerve Growth Factors/metabolism*
- Polymorphism, Single Nucleotide
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Synaptotagmins/genetics
- Synaptotagmins/metabolism
- Thyroid Dysgenesis/genetics*
- Tumor Suppressor Proteins/genetics
- Tumor Suppressor Proteins/metabolism*
- PubMed
- 25353184 Full text @ Endocrinology
Citation
Opitz, R., Hitz, M.P., Vandernoot, I., Trubiroha, A., Abu-Khudir, R., Samuels, M., Désilets, V., Costagliola, S., Andelfinger, G., Deladoëy, J. (2015) Functional Zebrafish Studies Based on Human Genotyping Point to Netrin-1 as a Link Between Aberrant Cardiovascular Development and Thyroid Dysgenesis. Endocrinology. 1(1):377-88.
Abstract
Congenital hypothyroidism caused by thyroid dysgenesis (CHTD) is a common congenital disorder with a birth prevalence of 1 case in 4000 live births, and up to 8 of individuals with CHTD have co-occurring congenital heart disease. Initially we found nine patients with cardiac and thyroid congenital disorders in our cohort of 158 CHTD patients. To enrich for a rare phenotype likely to be genetically simpler, we selected three patients with a ventricular septal defect for molecular studies. Then, to assess whether rare de novo copy number variants and coding mutations in candidate genes are a source of genetic susceptibility, we used a genome-wide single-nucleotide polymorphism array and Sanger sequencing to analyze blood DNA samples from selected patients with co-occurring CHTD a congenital heart disease. We found rare variants in all three patients, and we selected Netrin-1 as the biologically most plausible contributory factor for functional studies. In zebrafish, ntn1a and ntn1b were not expressed in thyroid tissue, but ntn1a was expressed in pharyngeal arch mesenchyme, and ntn1a-deficient embryos displayed defective aortic arch artery formation and abnormal thyroid morphogenesis. The functional activity of the thyroid in ntn1a-deficient larvae was, however, preserved. Phenotypic analysis of affected zebrafish indicates that abnormal thyroid morphogenesis resulted from a lack of proper guidance exerted by the dysplastic vasculature of ntn1a-deficient embryos. Hence, careful phenotyping of patients combined with molecular and functional studies in zebrafish identify Netrin-1 as a potential shared genetic factor for cardiac and thyroid congenital defects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping