PUBLICATION
Heterogeneously Expressed fezf2 Patterns Gradient Notch Activity in Balancing the Quiescence, Proliferation, and Differentiation of Adult Neural Stem Cells
- Authors
- Berberoglu, M.A., Dong, Z., Li, G., Zheng, J., Trejo Martinez, L.d.e.l. .C., Peng, J., Wagle, M., Reichholf, B., Petritsch, C., Li, H., Pleasure, S.J., Guo, S.
- ID
- ZDB-PUB-141018-8
- Date
- 2014
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 34: 13911-23 (Journal)
- Registered Authors
- Berberoglu, Michael, Dong, Zhiqiang, Guo, Su, Peng, Jisong, Wagle, Mahendra
- Keywords
- adult neurogenesis, hippocampus, radial glia, self-renewal, single-cell analysis, vivo morpholino
- MeSH Terms
-
- Adult Stem Cells/metabolism*
- Animals
- Animals, Genetically Modified
- Cell Differentiation/physiology*
- Cell Proliferation/physiology*
- DNA-Binding Proteins/biosynthesis*
- Female
- Gene Expression Regulation, Developmental
- Male
- Mice
- Mice, Transgenic
- Nerve Tissue Proteins/biosynthesis*
- Neural Stem Cells/metabolism*
- Neurogenesis/physiology
- Receptors, Notch/metabolism*
- Zebrafish
- PubMed
- 25319688 Full text @ J. Neurosci.
Citation
Berberoglu, M.A., Dong, Z., Li, G., Zheng, J., Trejo Martinez, L.d.e.l. .C., Peng, J., Wagle, M., Reichholf, B., Petritsch, C., Li, H., Pleasure, S.J., Guo, S. (2014) Heterogeneously Expressed fezf2 Patterns Gradient Notch Activity in Balancing the Quiescence, Proliferation, and Differentiation of Adult Neural Stem Cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34:13911-23.
Abstract
Balancing quiescence, self-renewal, and differentiation in adult stem cells is critical for tissue homeostasis. The underlying mechanisms, however, remain incompletely understood. Here we identify Fezf2 as a novel regulator of fate balance in adult zebrafish dorsal telencephalic neural stem cells (NSCs). Transgenic reporters show intermingled fezf2-GFP(hi) quiescent and fezf2-GFP(lo) proliferative NSCs. Constitutive or conditional impairment of fezf2 activity demonstrates its requirement for maintaining quiescence. Analyses of genetic chimeras reveal a dose-dependent role of fezf2 in NSC activation, suggesting that the difference in fezf2 levels directionally biases fate. Single NSC profiling coupled with genetic analysis further uncovers a fezf2-dependent gradient Notch activity that is high in quiescent and low in proliferative NSCs. Finally, fezf2-GFP(hi) quiescent and fezf2-GFP(lo) proliferative NSCs are observed in postnatal mouse hippocampus, suggesting possible evolutionary conservation. Our results support a model in which fezf2 heterogeneity patterns gradient Notch activity among neighbors that is critical to balance NSC fate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping