PUBLICATION
Kinesin-12 influences axonal growth during zebrafish neural development
- Authors
- Xu, M., Liu, D., Dong, Z., Wang, X., Wang, X., Liu, Y., Baas, P.W., Liu, M.
- ID
- ZDB-PUB-140925-5
- Date
- 2014
- Source
- Cytoskeleton (Hoboken, N.J.) 71(10): 555-63 (Journal)
- Registered Authors
- Liu, Yan
- Keywords
- axon, in situ hybridization, kinesin-12, microtubule, zebrafish
- MeSH Terms
-
- Animals
- Axons/metabolism*
- Conserved Sequence
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental/drug effects
- Humans
- In Situ Hybridization
- Kinesins/chemistry
- Kinesins/genetics
- Kinesins/metabolism*
- Morpholinos/pharmacology
- Nervous System/drug effects
- Nervous System/embryology*
- Nervous System/metabolism*
- Protein Structure, Tertiary
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins
- PubMed
- 25250533 Full text @ Cytoskeleton
Citation
Xu, M., Liu, D., Dong, Z., Wang, X., Wang, X., Liu, Y., Baas, P.W., Liu, M. (2014) Kinesin-12 influences axonal growth during zebrafish neural development. Cytoskeleton (Hoboken, N.J.). 71(10):555-63.
Abstract
Kinesin-12 (also called KIF15) is a microtubule-based motor protein best known for its role in cell division. We previously reported that kinesin-12 is robustly expressed in developing terminally post-mitotic neurons, with levels diminishing as neurons reach maturity. We found that axons of cultured rodent neurons grow faster and longer if kinesin-12 is experimentally depleted, leading us to conclude that kinesin-12 plays a role in modulating axonal growth. Here we used zebrafish to explore whether these results apply to an in vivo system and whether they apply across different kinds of vertebrates. In whole mount in situ hybridization, kinesin-12 mRNA was detectable at 2-cell and 1K-cell stages. At 5.3 and 8 hours post-fertilization (hpf), hybridization signal for kinesin-12 mRNA was observed in the ectoderm. From 14 to 36 hpf, the signal had expanded to the central nervous system. At 60 hpf, the hybridization signal was concentrated in the brain. After 5 days post-fertilization, kinesin-12 expression was reduced. Kinesin-12 knockdown resulted in notably longer fast-growing axons with fewer branches by injection of a splice-blocking morpholino into Tg(huC:egfp) or Tg(hb9:gfp) zebrafish embryos. Kinesin-12 overexpression resulted in shorter axons than controls. These results are consistent with our previous observations on rodents using primary cultures for the experimental manipulations, and suggest a key role of kinesin-12 as a modulator of axonal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping