PUBLICATION
Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic Ribbons
- Authors
- Sheets, L., Hagen, M.W., Nicolson, T.
- ID
- ZDB-PUB-140911-1
- Date
- 2014
- Source
- PLoS One 9: e107256 (Journal)
- Registered Authors
- Nicolson, Teresa, Sheets, Lavinia
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Eye Proteins/chemistry
- Eye Proteins/genetics*
- Eye Proteins/metabolism
- Gene Expression Regulation, Developmental
- Hair Cells, Auditory/metabolism*
- Hair Cells, Auditory/ultrastructure
- Larva/genetics
- Larva/growth & development
- Larva/metabolism
- Plasmids/metabolism
- Protein Structure, Tertiary
- Protein Subunits/chemistry
- Protein Subunits/genetics*
- Protein Subunits/metabolism
- Repressor Proteins/chemistry
- Repressor Proteins/genetics*
- Repressor Proteins/metabolism
- Synapses/metabolism*
- Synapses/ultrastructure
- Synaptic Transmission
- Transfection
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 25208216 Full text @ PLoS One
Citation
Sheets, L., Hagen, M.W., Nicolson, T. (2014) Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic Ribbons. PLoS One. 9:e107256.
Abstract
Synaptic ribbons are presynaptic structures formed by the self-association of RIBEYE-the main structural component of ribbon synapses. RIBEYE consists of two domains: a unique N-terminal A-domain and a C-terminal B-domain that is identical to the transcription co-repressor C-terminal binding protein 2 (CtBP2). Previous studies in cell lines have shown that RIBEYE A-domain alone is sufficient to form ribbon-like aggregates and that both A- and B- domains form homo-and heterotypic interactions. As these interactions are likely the basis for synaptic-ribbon assembly and structural plasticity, we wanted to examine how zebrafish Ribeye A- and B- domains interact with synaptic ribbons in vivo. To that end, we characterized the localization of exogenously expressed Ribeye A- and B- domains and the closely related protein, CtBP1, in the hair cells of transgenic zebrafish larvae. Unexpectedly, exogenously expressed Ribeye A-domain showed variable patterns of localization in hair cells; one zebrafish paralog of A-domain failed to self-associate or localize to synaptic ribbons, while the other self-assembled but sometimes failed to localize to synaptic ribbons. By contrast, Ribeye B-domain/CtBP2 was robustly localized to synaptic ribbons. Moreover, both exogenously expressed B-domain/CtBP2 and CtBP1 were preferentially localized to the basal end of ribbons adjacent to the postsynaptic density. Overexpression of B-domain/CtBP2 also appeared to affect synaptic-ribbon composition; endogenous levels of ribbon-localized Ribeye were significantly reduced as hair cells matured in B-domain/CtBP2 transgenic larvae compared to wild-type. These results reveal how exogenously expressed Ribeye domains interact with synaptic ribbons, and suggest a potential organization of elements within the ribbon body.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping