PUBLICATION

Zinc finger MYND-type containing 8 promotes tumour angiogenesis via induction of vascular endothelial growth factor-A expression

Authors
Kuroyanagi, J., Shimada, Y., Zhang, B., Ariyoshi, M., Umemoto, N., Nishimura, Y., Tanaka, T.
ID
ZDB-PUB-140815-11
Date
2014
Source
FEBS letters   588(18): 3409-16 (Journal)
Registered Authors
Tanaka, Toshio
Keywords
DNA microarray, Prostate cancer, Tumour vessels, Xenotransplantation, Zebrafish
MeSH Terms
  • Animals
  • Cell Line, Tumor
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques
  • Human Umbilical Vein Endothelial Cells/physiology
  • Humans
  • Male
  • Neoplasm Transplantation
  • Neovascularization, Pathologic/genetics
  • Neovascularization, Pathologic/metabolism*
  • Receptors, Cell Surface/physiology*
  • Transcriptional Activation*
  • Transcriptome
  • Vascular Endothelial Growth Factor A/genetics*
  • Vascular Endothelial Growth Factor A/metabolism
  • Zebrafish
PubMed
25117453 Full text @ FEBS Lett.
Abstract
Zinc finger, MYND-type containing 8 (ZMYND8) encodes a receptor for activated C-kinase protein. Here, we report that ZMYND8 promotes angiogenesis in prostate cancer xenografts in zebrafish, as well as tube formation in human umbilical vascular endothelial cell (HUVEC) cultures. Using transcriptome analyses, we found upregulation of ZMYND8 expression in both zebrafish prostate cancer xenografts and prostate cancer samples from patients. In vitroo and in vivo ZMYND8 knockdown suppressed angiogenesis, whereas ZMYND8 overexpression enhanced angiogenesis. Notably, ZMYND8 induced vegfa mRNA expression selectively in prostate cancer xenografts. Integrated analysis of human and zebrafish transcriptomes, which identified ZMYND8, might be a powerful strategy to determine also other molecular targets for inhibiting prostate cancer progression.
Errata / Notes
Corrections: ZDB-PUB-160213-11
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping