PUBLICATION
High-resolution reconstruction of the beating zebrafish heart
- Authors
- Mickoleit, M., Schmid, B., Weber, M., Fahrbach, F.O., Hombach, S., Reischauer, S., Huisken, J.
- ID
- ZDB-PUB-140722-18
- Date
- 2014
- Source
- Nature Methods 11(9): 919-22 (Journal)
- Registered Authors
- Hombach, Sonja, Huisken, Jan, Mickoleit, Michaela, Reischauer, Sven
- Keywords
- none
- MeSH Terms
-
- Algorithms
- Animals
- Cell Tracking/methods*
- Image Enhancement/methods*
- Image Interpretation, Computer-Assisted/methods*
- Imaging, Three-Dimensional/methods*
- Microscopy, Video/methods*
- Myocytes, Cardiac/cytology*
- Myocytes, Cardiac/physiology
- Reproducibility of Results
- Sensitivity and Specificity
- Subtraction Technique
- Zebrafish/anatomy & histology*
- Zebrafish/physiology
- PubMed
- 25042787 Full text @ Nat. Methods
Citation
Mickoleit, M., Schmid, B., Weber, M., Fahrbach, F.O., Hombach, S., Reischauer, S., Huisken, J. (2014) High-resolution reconstruction of the beating zebrafish heart. Nature Methods. 11(9):919-22.
Abstract
The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping