PUBLICATION
Aciculin interacts with filamin C and Xin and is essential for myofibril assembly, remodeling and maintenance
- Authors
- Molt, S., Bührdel, J.B., Yakovlev, S., Schein, P., Orfanos, Z., Kirfel, G., Winter, L., Wiche, G., van der Ven, P.F., Rottbauer, W., Just, S., Belkin, A.M., Fürst, D.O.
- ID
- ZDB-PUB-140626-2
- Date
- 2014
- Source
- Journal of Cell Science 127(Pt 16): 3578-92 (Journal)
- Registered Authors
- Bührdel, John, Just, Steffen, Rottbauer, Wolfgang
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Cells, Cultured
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism*
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Filamins/genetics
- Filamins/metabolism*
- Humans
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Myoblasts/metabolism
- Myofibrils/genetics
- Myofibrils/metabolism*
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism*
- Phosphoglucomutase/genetics
- Phosphoglucomutase/metabolism*
- Protein Binding
- PubMed
- 24963132 Full text @ J. Cell Sci.
Citation
Molt, S., Bührdel, J.B., Yakovlev, S., Schein, P., Orfanos, Z., Kirfel, G., Winter, L., Wiche, G., van der Ven, P.F., Rottbauer, W., Just, S., Belkin, A.M., Fürst, D.O. (2014) Aciculin interacts with filamin C and Xin and is essential for myofibril assembly, remodeling and maintenance. Journal of Cell Science. 127(Pt 16):3578-92.
Abstract
Filamin C (FLNc) and Xin actin-binding repeat-containing proteins (XIRPs) are multi-adapter proteins mainly expressed in cardiac and skeletal muscles that play important roles in the assembly and repair of myofibrils and their attachment to the membrane. We identified the dystrophin-binding protein aciculin (PGM5), as a novel interaction partner of FLNc and Xin. All three proteins colocalize at intercalated discs of cardiac muscle and myotendinous junctions of skeletal muscle, while FLNc and aciculin also colocalize in mature Z-discs. Bimolecular fluorescence complementation experiments in developing cultured mammalian skeletal muscle cells demonstrate that Xin and aciculin also interact in FLNc-containing immature myofibrils and areas of myofibrillar remodeling and repair induced by electrical pulse stimulation (EPS). FRAP experiments show that aciculin is a highly dynamic and mobile protein. Aciculin knockdown in myotubes leads to failure in myofibril assembly, alignment and membrane attachment, and massive reduction in myofibril number. A highly similar phenotype was found upon depletion of aciculin in zebrafish embryos. Our results point to a thus far unappreciated but essential function of aciculin in myofibril formation, maintenance and remodeling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping