PUBLICATION
Differential reparative phenotypes between zebrafish and medaka after cardiac injury
- Authors
- Ito, K., Morioka, M., Kimura, S., Tasaki, M., Inohaya, K., Kudo, A.
- ID
- ZDB-PUB-140621-10
- Date
- 2014
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 243(9): 1106-15 (Journal)
- Registered Authors
- Kudo, Akira
- Keywords
- heart regeneration, heart repair, medaka, periostin, raldh2, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Proliferation
- Heart/physiology*
- Heart Injuries/physiopathology*
- Myocardium/metabolism
- Oryzias
- Phenotype
- Regeneration/physiology*
- Zebrafish
- PubMed
- 24947076 Full text @ Dev. Dyn.
Citation
Ito, K., Morioka, M., Kimura, S., Tasaki, M., Inohaya, K., Kudo, A. (2014) Differential reparative phenotypes between zebrafish and medaka after cardiac injury. Developmental Dynamics : an official publication of the American Association of Anatomists. 243(9):1106-15.
Abstract
Background Zebrafish have the ability for heart regeneration. However, another teleost animal model, the medaka, had not yet been investigated for this capacity.
Results Compared with zebrafish, the medaka heart responded differently to an injury: An excessive fibrotic response occurred in the medaka heart, and existing cardiomyocytes or cardiac progenitor cells remained dormant, resulting in no numerical difference between the uncut and injured heart with respect to the number of EdU-incorporated cardiomyocytes. The results obtained from the analysis of the medaka raldh2-GFP transgenic line showed a lack of raldh2 expression in the endocardium. Regarding periostin expression, the localization of medaka periostin-b, a marker of fibrillogenesis, in the medaka heart remained at the wound site at 30 dpa; whereas zebrafish periostin-b was no longer localized at the wound but was detected in the epicardium at that time.
Conclusions Compared with zebrafish heart regeneration, the medaka heart phenotypes suggest the possibility that the medaka could hardly regenerate its heart tissue or that these phenotypes for heart regeneration showed a delay.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping