PUBLICATION
Hypoxia-inducible factor-1 mediates adaptive developmental plasticity of hypoxia tolerance in zebrafish, Danio rerio
- Authors
- Robertson, C.E., Wright, P.A., Köblitz, L., Bernier, N.J.
- ID
- ZDB-PUB-140523-1
- Date
- 2014
- Source
- Proceedings. Biological sciences 281(1786): 882-98 (Journal)
- Registered Authors
- Keywords
- Hypoxia-inducible factor-1 cellular pathway, developmental plasticity, hypoxia tolerance, sex ratio bias, zebrafish
- MeSH Terms
-
- Anaerobiosis*
- Animals
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/physiology
- Female
- Hypoxia-Inducible Factor 1/genetics*
- Hypoxia-Inducible Factor 1/metabolism
- Larva/genetics
- Larva/growth & development
- Larva/physiology
- Male
- Oxygen/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/physiology*
- PubMed
- 24850928 Full text @ Proc. Biol. Sci.
Citation
Robertson, C.E., Wright, P.A., Köblitz, L., Bernier, N.J. (2014) Hypoxia-inducible factor-1 mediates adaptive developmental plasticity of hypoxia tolerance in zebrafish, Danio rerio. Proceedings. Biological sciences. 281(1786):882-98.
Abstract
In recent years, natural and anthropogenic factors have increased aquatic hypoxia the world over. In most organisms, the cellular response to hypoxia is mediated by the master regulator hypoxia-inducible factor-1 (HIF-1). HIF-1 also plays a critical role in the normal development of the cardiovascular system of vertebrates. We tested the hypothesis that hypoxia exposures which resulted in HIF-1 induction during embryogenesis would be associated with enhanced hypoxia tolerance in subsequent developmental stages. We exposed zebrafish (Danio rerio) embryos to just 4 h of severe hypoxia or total anoxia at 18, 24 and 36 h post-fertilization (hpf). Of these, exposure to hypoxia at 24 and 36 hpf as well as anoxia at 36 hpf activated the HIF-1 cellular pathway. Zebrafish embryos that acutely upregulated the HIF-1 pathway had an increased hypoxia tolerance as larvae. The critical window for hypoxia sensitivity and HIF-1 signalling was 24 hpf. Adult male fish had a lower critical oxygen tension (Pcrit) compared with females. Early induction of HIF-1 correlated directly with an increased proportion of males in the population. We conclude that mounting a HIF-1 response during embryogenesis is associated with long-term impacts on the phenotype of later stages which could influence both individual hypoxia tolerance and population dynamics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping