PUBLICATION
Multilayer mounting for long-term light sheet microscopy of zebrafish
- Authors
- Weber, M., Mickoleit, M., Huisken, J.
- ID
- ZDB-PUB-140513-335
- Date
- 2014
- Source
- Journal of visualized experiments : JoVE (84): e51119 (Journal)
- Registered Authors
- Huisken, Jan, Mickoleit, Michaela
- Keywords
- none
- MeSH Terms
-
- Animals
- Female
- Male
- Microscopy/instrumentation*
- Microscopy/methods*
- Sepharose
- Time-Lapse Imaging/instrumentation*
- Time-Lapse Imaging/methods*
- Zebrafish/embryology*
- PubMed
- 24637614 Full text @ J. Vis. Exp.
Citation
Weber, M., Mickoleit, M., Huisken, J. (2014) Multilayer mounting for long-term light sheet microscopy of zebrafish. Journal of visualized experiments : JoVE. (84):e51119.
Abstract
Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping