PUBLICATION
Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation
- Authors
- Balasubramanian, A., Kawahara, G., Gupta, V.A., Rozkalne, A., Beauvais, A., Kunkel, L.M., Gussoni, E.
- ID
- ZDB-PUB-140513-236
- Date
- 2014
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28(7): 2955-2969 (Journal)
- Registered Authors
- Gupta, Vandana A, Kawahara, Genri, Kunkel, Louis M.
- Keywords
- acetylation, muscular dystrophy, myoblast, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Differentiation/genetics*
- Cells, Cultured
- Down-Regulation/genetics
- Histone Deacetylases/genetics
- Histone Deacetylases/metabolism*
- Humans
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Muscle Cells/metabolism*
- Muscle Development/genetics*
- Muscle Proteins/genetics
- Muscle Proteins/metabolism*
- Muscle, Skeletal/metabolism
- Muscular Diseases/genetics
- Muscular Diseases/metabolism
- Protein Binding/genetics
- Proteins/genetics*
- Proteins/metabolism*
- Sequence Alignment
- Tubulin/genetics
- Tubulin/metabolism
- Zebrafish
- PubMed
- 24687993 Full text @ FASEB J.
Citation
Balasubramanian, A., Kawahara, G., Gupta, V.A., Rozkalne, A., Beauvais, A., Kunkel, L.M., Gussoni, E. (2014) Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(7):2955-2969.
Abstract
Previously, we identified family with sequence similarity 65, member B (Fam65b), as a protein transiently up-regulated during differentiation and fusion of human myogenic cells. Silencing of Fam65b expression results in severe reduction of myogenin expression and consequent lack of myoblast fusion. The molecular function of Fam65b and whether misregulation of its expression could be causative of muscle diseases are unknown. Protein pulldowns were used to identify Fam65b-interacting proteins in differentiating human muscle cells and regenerating muscle tissue. In vitro, human muscle cells were treated with histone-deacetylase (HDAC) inhibitors, and expression of Fam65b and interacting proteins was studied. Nontreated cells were used as controls. In vivo, expression of Fam65b was down-regulated in developing zebrafish to determine the effects on muscle development. Fam65b binds to HDAC6 and dysferlin, the protein mutated in limb girdle muscular dystrophy 2B. The tricomplex Fam65b-HDAC6-dysferlin is transient, and Fam65b expression is necessary for the complex to form. Treatment of myogenic cells with pan-HDAC or HDAC6-specific inhibitors alters Fam65b expression, while dysferlin expression does not change. Inhibition of Fam65b expression in developing zebrafish results in abnormal muscle, with low birefringence, tears at the myosepta, and increased embryo lethality. Fam65b is an essential component of the HDAC6-dysferlin complex. Down-regulation of Fam65b in developing muscle causes changes consistent with muscle disease.-Balasubramanian, A., Kawahara, G., Gupta, V. A., Rozkalne, A., Beauvais, A., Kunkel, L. M., Gussoni, E. Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping