PUBLICATION
Effects of 6-Hydroxydopamine Exposure on Motor Activity and Biochemical Expression in Zebrafish (Danio Rerio) Larvae
- Authors
- Feng, C.W., Wen, Z.H., Huang, S.Y., Hung, H.C., Chen, C.H., Yang, S.N., Chen, N.F., Wang, H.M., Hsiao, C.D., Chen, W.F.
- ID
- ZDB-PUB-140513-167
- Date
- 2014
- Source
- Zebrafish 11(3): 227-39 (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- none
- MeSH Terms
-
- Animals
- Antiparkinson Agents/administration & dosage
- Antiparkinson Agents/pharmacology
- Anxiety/chemically induced
- Biomarkers/metabolism
- Blotting, Western
- Carbidopa/administration & dosage
- Carbidopa/pharmacology
- Disease Models, Animal
- Drug Combinations
- Levodopa/administration & dosage
- Levodopa/pharmacology
- Minocycline/administration & dosage
- Minocycline/pharmacology
- Motor Activity/drug effects
- Oxidopamine/pharmacology*
- Parkinsonian Disorders/chemically induced
- Parkinsonian Disorders/drug therapy
- Parkinsonian Disorders/physiopathology*
- Real-Time Polymerase Chain Reaction
- Sympatholytics/pharmacology*
- Vitamin E/administration & dosage
- Vitamin E/pharmacology
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 24720843 Full text @ Zebrafish
Citation
Feng, C.W., Wen, Z.H., Huang, S.Y., Hung, H.C., Chen, C.H., Yang, S.N., Chen, N.F., Wang, H.M., Hsiao, C.D., Chen, W.F. (2014) Effects of 6-Hydroxydopamine Exposure on Motor Activity and Biochemical Expression in Zebrafish (Danio Rerio) Larvae. Zebrafish. 11(3):227-39.
Abstract
Abstract Parkinson's disease (PD) is a neurodegenerative disease that is characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra. However, current treatments for PD are mainly palliative. Recently, researchers discovered that neurotoxins can induce Parkinsonian-like symptoms in zebrafish. No study to date has investigated the characteristics of PD, such as neuroinflammation factors, oxidative stress, or ubiquitin dysfunction, in this model. Therefore, the current study was aimed at utilizing commonly used clinical drugs, minocycline, vitamin E, and Sinemet, to test the usefulness of this model. Previous studies had indicated that DA cell loss was greater with 6-hydroxydopamine (6-OHDA) than with other neurotoxins. Thus, we first challenged zebrafish with 6-OHDA immersion and found a significant reduction in zebrafish locomotor activity; we then reversed the locomotor disruptions by treatment with vitamin E, Sinemet, or minocycline. The present study also analyzed the mRNA expression of parkin, pink1, and cd-11b, because the expression of these molecular targets has been shown to result in attenuation in mammalian models of PD. Vitamin E, Sinemet, and minocycline significantly reversed 6-OHDA-induced changes of parkin, pink1, and cd-11b mRNA expression in zebrafish. Moreover, we assessed tyrosine hydroxylase (TH) expression to confirm the therapeutic effects of vitamin E tested on this PD model and established that vitamin E reversed the 6-OHDA-induced damage on TH expression. Our results provide some support for the validity of this in vivo Parkinson's model, and we hope that this model will be more widely used in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping