PUBLICATION
Rapid adaptive optical recovery of optimal resolution over large volumes
- Authors
- Wang, K., Milkie, D.E., Saxena, A., Engerer, P., Misgeld, T., Bronner, M.E., Mumm, J., Betzig, E.
- ID
- ZDB-PUB-140513-151
- Date
- 2014
- Source
- Nature Methods 11(6): 625-8 (Journal)
- Registered Authors
- Bronner-Fraser, Marianne, Mumm, Jeff, Saxena, Ankur
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Microscopy, Confocal/instrumentation*
- Microscopy, Confocal/statistics & numerical data*
- Optics and Photonics/instrumentation*
- Optics and Photonics/standards
- Time Factors
- Zebrafish
- PubMed
- 24727653 Full text @ Nat. Methods
Citation
Wang, K., Milkie, D.E., Saxena, A., Engerer, P., Misgeld, T., Bronner, M.E., Mumm, J., Betzig, E. (2014) Rapid adaptive optical recovery of optimal resolution over large volumes. Nature Methods. 11(6):625-8.
Abstract
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping