PUBLICATION

Knockdown of zebrafish blood vessel epicardial substance results in incomplete retinal lamination

Authors
Wu, Y.C., Chen, R.F., Liu, C.Y., Hu, F.R., Huang, C.J., Wang, I.J.
ID
ZDB-PUB-140513-130
Date
2014
Source
TheScientificWorldJournal   2014: 803718 (Journal)
Registered Authors
Huang, Chang-Jen
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cell Polarity
  • DNA Primers
  • Retina/cytology*
  • Retinal Vessels/metabolism*
  • Zebrafish*
PubMed
24741362 Full text @ ScientificWorldJournal
Abstract
Cell polarity during eye development determines the normal retinal lamination and differentiation of photoreceptor cells in the retina. In vertebrates, blood vessel epicardial substance (Bves) is known to play an important role in the formation and maintenance of the tight junctions essential for epithelial cell polarity. In the current study, we generated a transgenic zebrafish Bves (zbves) promoter-EGFP zebrafish line to investigate the expression pattern of Bves in the retina and to study the role of zbves in retinal lamination. Immunostaining with different specific antibodies from retinal cells and transmission electron microscopy were used to identify the morphological defects in normal and Bves knockdown zebrafish. In normal zebrafish, Bves is located at the apical junctions of embryonic retinal neuroepithelia during retinogenesis; later, it is strongly expressed around inner plexiform layer (IPL) and retinal pigment epithelium (RPE). In contrast, a loss of normal retinal lamination and cellular polarity was found with undifferentiated photoreceptor cells in Bves knockdown zebrafish. Herein, our results indicated that disruption of Bves will result in a loss of normal retinal lamination.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping