PUBLICATION
Analyzing craniofacial morphogenesis in zebrafish using 4D confocal microscopy
- Authors
- McGurk, P.D., Lovely, C.B., and Eberhart, J.K.
- ID
- ZDB-PUB-140429-9
- Date
- 2014
- Source
- Journal of visualized experiments : JoVE (83): e51190 (Journal)
- Registered Authors
- Eberhart, Johann
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Craniofacial Abnormalities/genetics
- Facial Bones/embryology*
- Microscopy, Confocal/methods*
- Morphogenesis/genetics
- Morphogenesis/physiology
- Neural Crest/embryology
- Receptor, Platelet-Derived Growth Factor alpha/genetics
- Receptors, G-Protein-Coupled/genetics
- Smad5 Protein/genetics
- Time-Lapse Imaging/methods
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 24514435 Full text @ J. Vis. Exp.
Citation
McGurk, P.D., Lovely, C.B., and Eberhart, J.K. (2014) Analyzing craniofacial morphogenesis in zebrafish using 4D confocal microscopy. Journal of visualized experiments : JoVE. (83):e51190.
Abstract
Time-lapse confocal imaging is a powerful technique useful for characterizing embryonic development. Here, we describe the methodology and characterize craniofacial morphogenesis in wild-type, as well as pdgfra, smad5, and smo mutant embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping