PUBLICATION

Interplay between autophagy and apoptosis in the development of Danio rerio follicles and the effects of a probiotic

Authors
Gioacchini, G., Valle, L.D., Benato, F., Fimia, G.M., Nardacci, R., Ciccosanti, F., Piacentini, M., Borini, A., and Carnevali, O.
ID
ZDB-PUB-140218-1
Date
2013
Source
Reproduction, fertility, and development   25(8): 1115-1125 (Journal)
Registered Authors
Benato, Francesca, Carnevali, Oliana, Piacentini, Mauro
Keywords
none
MeSH Terms
  • Animals
  • Apoptosis*
  • Apoptosis Regulatory Proteins/antagonists & inhibitors
  • Apoptosis Regulatory Proteins/genetics
  • Apoptosis Regulatory Proteins/metabolism
  • Autophagy*
  • Diet/veterinary*
  • Female
  • Gene Expression Regulation, Developmental
  • Insulin-Like Growth Factor I
  • Insulin-Like Growth Factor II/biosynthesis
  • Insulin-Like Growth Factor II/genetics
  • Insulin-Like Growth Factor II/metabolism
  • Lacticaseibacillus rhamnosus/growth & development*
  • Microscopy, Electron, Transmission
  • Microtubule-Associated Proteins/biosynthesis
  • Microtubule-Associated Proteins/genetics
  • Microtubule-Associated Proteins/metabolism
  • Oogenesis
  • Ovarian Follicle/cytology*
  • Ovarian Follicle/metabolism
  • Ovarian Follicle/ultrastructure
  • Probiotics*
  • Proto-Oncogene Proteins c-myc/antagonists & inhibitors
  • Proto-Oncogene Proteins c-myc/genetics
  • Proto-Oncogene Proteins c-myc/metabolism
  • RNA, Messenger/metabolism
  • Somatomedins/biosynthesis
  • Somatomedins/genetics
  • Somatomedins/metabolism
  • Vitellogenesis
  • Zebrafish/growth & development*
  • Zebrafish/microbiology
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
23195281 Full text @ Reprod. Fertil. Dev.
Abstract

The present study investigated autophagic processes in Danio rerio preovulatory follicles (Stage III and IV). There were more autophagosomes, as revealed by electron microscopy, in follicles from females fed the probiotic Lactobacillus rhamnosus IMC 501. This was confirmed by increased expression of genes involved in the autophagic process, namely ambra1, becn1, lc3 and uvrag. In addition, preovulatory follicles from females fed the probiotic contained more microtubule-associated protein 1 light chain 3 isoform II (LC3-II) and less p62 protein. The increased autophagy in preovulatory follicles from females fed the probiotic was concomitant with a decrease in the apoptotic process in the ovary, as evidenced by terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling analysis and confirmed by lower expression of genes involved in apoptosis (i.e. p53, bax, apaf and cas3) and higher expression as igfII and igf1r. The results of the present study provide preliminary evidence of the involvement of autophagy during follicle development in the zebrafish ovary. In addition, we have demonstrated for the first time that a functional food, such as L. rhamnosus IMC 501, can modulate the balance between apoptosis and autophagy that regulates ovary physiology in zebrafish by inhibiting follicular apoptosis and improving follicular survival.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping