PUBLICATION

Evolutionary conservation of early mesoderm specification by mechanotransduction in Bilateria

Authors
Brunet, T., Bouclet, A., Ahmadi, P., Mitrossilis, D., Driquez, B., Brunet, A.C., Henry, L., Serman, F., Béalle, G., Ménager, C., Dumas-Bouchiat, F., Givord, D., Yanicostas, C., Le-Roy, D., Dempsey, N.M., Plessis, A., and Farge, E.
ID
ZDB-PUB-140113-23
Date
2013
Source
Nature communications   4: 2821 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Armadillo Domain Proteins/metabolism*
  • Biological Evolution*
  • Conserved Sequence/physiology
  • Drosophila
  • Drosophila Proteins/metabolism*
  • Female
  • Fetal Proteins
  • Male
  • Mechanotransduction, Cellular*/physiology
  • Mesoderm/physiology*
  • Signal Transduction/physiology
  • T-Box Domain Proteins/metabolism
  • Transcription Factors/metabolism*
  • Twist-Related Protein 1/metabolism
  • Zebrafish
  • Zebrafish Proteins/metabolism*
  • beta Catenin/metabolism*
PubMed
24281726 Full text @ Nat. Commun.
Abstract

The modulation of developmental biochemical pathways by mechanical cues is an emerging feature of animal development, but its evolutionary origins have not been explored. Here we show that a common mechanosensitive pathway involving β-catenin specifies early mesodermal identity at gastrulation in zebrafish and Drosophila. Mechanical strains developed by zebrafish epiboly and Drosophila mesoderm invagination trigger the phosphorylation of β-catenin–tyrosine-667. This leads to the release of β-catenin into the cytoplasm and nucleus, where it triggers and maintains, respectively, the expression of zebrafish brachyury orthologue notail and of Drosophila Twist, both crucial transcription factors for early mesoderm identity. The role of the β-catenin mechanosensitive pathway in mesoderm identity has been conserved over the large evolutionary distance separating zebrafish and Drosophila. This suggests mesoderm mechanical induction dating back to at least the last bilaterian common ancestor more than 570 million years ago, the period during which mesoderm is thought to have emerged.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping