PUBLICATION

Phosphorylation of Dpsyl2 (CRMP2) and Dpsyl3 (CRMP4) is required for positioning of caudal primary motor neurons in the zebrafish spinal cord

Authors
Morimura, R., Nozawa, K., Tanaka, H., and Ohshima, T.
ID
ZDB-PUB-130903-7
Date
2013
Source
Developmental Neurobiology   73(12): 911-20 (Journal)
Registered Authors
Tanaka, Hideomi
Keywords
motor neuron, spinal cord, positioning, phosphorylation, zebrafish
MeSH Terms
  • Protein Serine-Threonine Kinases/genetics
  • Protein Serine-Threonine Kinases/metabolism
  • Gene Expression Regulation, Developmental/genetics*
  • Spinal Cord/metabolism*
  • Protein-Tyrosine Kinases/genetics
  • Protein-Tyrosine Kinases/metabolism
  • Semaphorin-3A/genetics
  • Semaphorin-3A/metabolism
  • Phosphorylation/physiology
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals
  • Motor Neurons/metabolism*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Cyclin-Dependent Kinase 5/genetics
  • Cyclin-Dependent Kinase 5/metabolism
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism*
  • Gene Knockdown Techniques/methods
PubMed
23929741 Full text @ Dev. Neurobiol.
Abstract

Dpysls (CRMPs) that were initially identified as mediator proteins of Semaphorin3a (Sem3a) signaling are involved in neuronal polarity and axon elongation in cultured neurons. Previous studies have shown that knockdown of neuropilin1a, one of the sem3a receptors, exhibited ectopic primary motor neurons (PMNs) outside of the spinal cord in zebrafish. However, downstream molecules of sem3a signaling involved in the positioning of motor neurons are largely unknown. Here, we addressed the role of Dpysl2 (CRMP2) and Dpysl3 (CRMP4) in the positioning of PMNs in the zebrafish spinal cord. We found that the knockdown of dpysls by antisense morpholino oligonucleotides (AMO) causes abnormal positioning of caudal primary (CaP) motor neurons outside the spinal cord. The knockdown of cdk5 and dyrk2 by AMO also caused similar phenotype in the positioning of CaP motor neurons, and this phenotype was rescued by co-injection of phosphorylation-mimic type dpysl2 mRNA. These results suggest that the phosphorylation of Dpysl2 and Dpysl3 by Cdk5 and Dyrk2 is required for correct positioning of CaP motor neurons in the zebrafish spinal cord.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping