Proteomic and functional analysis of zebrafish after administration of antimicrobial peptide epinecidin-1
- Authors
- Huang, T.C., and Chen, J.Y.
- ID
- ZDB-PUB-121227-13
- Date
- 2013
- Source
- Fish & shellfish immunology 34(2): 593-598 (Journal)
- Registered Authors
- Chen, Jyh-Yih
- Keywords
- proteomic, zebrafish, epinecidin-1, antimicrobial peptide
- MeSH Terms
-
- Animals
- Antimicrobial Cationic Peptides/administration & dosage
- Antimicrobial Cationic Peptides/pharmacology*
- Cytoskeleton/drug effects
- Cytoskeleton/physiology*
- DNA Primers/genetics
- Electrophoresis, Gel, Two-Dimensional/veterinary
- Fish Proteins/administration & dosage
- Fish Proteins/pharmacology*
- Gene Expression Regulation/drug effects*
- Immunity, Innate/immunology*
- Male
- Mass Spectrometry/veterinary
- Proteomics/methods
- Real-Time Polymerase Chain Reaction/veterinary
- Reverse Transcriptase Polymerase Chain Reaction/veterinary
- Zebrafish/immunology*
- Zebrafish/metabolism
- PubMed
- 23261508 Full text @ Fish Shellfish Immunol.
Antimicrobial peptides (AMPs) play important roles in innate immunity. One such AMP, epinecidin-1, exhibits antibacterial effects in zebrafish. In the current study, we aimed to identify the antimicrobial-associated proteins affected by epinecidin-1 treatment, and to unravel the underlying antimicrobial molecular mechanisms of epinecidin-1. We analyzed proteome changes in epinecidin-1-treated zebrafish using two-dimensional electrophoresis (2DE) coupled to mass spectrometry. Several differentially expressed proteins were identified, some of which were validated by real-time quantitative RT-PCR. The differentially expressed proteins were mapped onto Ingenuity Pathway Analysis canonical pathways, to construct a possible protein–protein interacting network regulated by epinecidin-1; this network suggested a potential role of epinecindin-1 in cytoskeletal assembly and organization. Our findings imply that epinecidin-1 may stabilize the cytoskeleton network in host cells, thereby promoting resistance to bacterial infection.