Slc45a2 and V-ATPase are regulators of melanosomal pH homeostasis in zebrafish, providing a mechanism for human pigment evolution and disease
- Authors
- Dooley, C.M., Schwarz, H., Mueller, K.P., Mongera, A., Konantz, M., Neuhauss, S.C., Nüsslein-Volhard, C., and Geisler, R.
- ID
- ZDB-PUB-121206-42
- Date
- 2013
- Source
- Pigment cell & melanoma research 26(2): 205-217 (Journal)
- Registered Authors
- Dooley, Christopher, Geisler, Robert, Konantz, Martina, Mongera, Alessandro, Neuhauss, Stephan, Nüsslein-Volhard, Christiane
- Keywords
- slc45a2, albinism, v-ATPase, melanosome, melanocyte, retinal pigment epithelium, skin color variation
- MeSH Terms
-
- Hydrogen-Ion Concentration/drug effects
- Protein Subunits/chemistry
- Protein Subunits/metabolism
- Amino Acid Sequence
- Organ Specificity/drug effects
- Melanocytes/metabolism
- Melanocytes/pathology
- Homeostasis*/drug effects
- Models, Biological
- Molecular Sequence Data
- Zebrafish/metabolism*
- Melanophores/drug effects
- Melanophores/metabolism
- Visual Acuity/drug effects
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/metabolism*
- Humans
- Cloning, Molecular
- Pigmentation*/drug effects
- Biological Evolution*
- Alleles
- Membrane Transport Proteins/chemistry
- Membrane Transport Proteins/metabolism*
- Melanosomes/drug effects
- Melanosomes/metabolism*
- Melanosomes/ultrastructure
- Neural Crest/drug effects
- Neural Crest/metabolism
- Neural Crest/pathology
- Animals
- Monophenol Monooxygenase/metabolism
- Vacuolar Proton-Translocating ATPases/antagonists & inhibitors
- Vacuolar Proton-Translocating ATPases/metabolism*
- Protein Structure, Tertiary
- Retina/drug effects
- Retina/metabolism
- Retina/pathology
- Morpholinos/pharmacology
- Mutation/genetics
- PubMed
- 23205854 Full text @ Pigment Cell Melanoma Res.
We present here the positional cloning of the Danio rerio albino mutant and show that the affected gene encodes Slc45a2. The human orthologous gene has previously been shown to be involved in human skin color variation and mutations therein have been implicated in the disease OCA 4. Through ultrastructural analysis of the melanosomes in albino alleles as well as the tyrosinase deficient mutant sandy we add new insights into the role of Slc45a2 in the production of melanin. In order to gain further understanding of the role of Slc45a2 and its possible interactions with other proteins involved in melanization we further analyzed the role of the V-ATPase as a melanosomal acidifier. We show that it is possible to rescue the melanization potential of the albino melanosomes through genetic and chemical inhibition of V-ATPase thereby increasing internal melanosome pH.